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Rapid ID of yeasts by gene sequences

Domains 1 and 2 (D1/D2) of LSU rDNA. Easy to PCR (universal primers), sequence, align… Short (400-650 bp) but variable enough to distinguish most of yeast species Universally available database for all known yeast species Kurtzman and Robnett (1998) - ascomycete yeasts

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Rapid ID of yeasts by gene sequences

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  1. Domains 1 and 2 (D1/D2) of LSU rDNA • Easy to PCR (universal primers), sequence, align… • Short (400-650 bp) but variable enough to • distinguish most of yeast species • Universally available database for all known yeast species • Kurtzman and Robnett (1998) - ascomycete yeasts • Fell et al. (2000) - basidiomycete yeasts Rapid ID of yeasts by gene sequences

  2. Correlation of nDNA relatedness and D1/D2 sequence divergence among ascomycetous yeasts Strain pairs % nDNA D1/D2 nucleotide relatedness differences Conspecific >70% 0-3 Unrelated <20% 6-250 Kurtzman 2006, Mycoscience 47: 65-71

  3. D1/D2 sequencing Database 686 strains - Known yeasts 81 ascomycetous spp. 39 basidiomycetous spp. 1241 strains - New yeasts 214 ascomycete spp. 31 basidiomycete spp. 18S, ITS, phenotypic characters 618 strains - Described as 66 novel ascomycete spp. 1927 yeast strains from insect guts

  4. C Is single gene enough for ID? B A

  5. Other genes • ITS - widely, useful for closely related taxa • cytochrome oxidase II - Belloch et al. 2000 • actin-1 - Daniel et al. 2001 • EF1-a, RPB II, mSSU - Kurtzman & Robnett 2003, 2007

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