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V 2.01

V 2.01. In the heart of hematopoiesis. ABX PENTRA DX 120. Main features analysis Measurement principles Expert Validation Station SPS Evolution. External view. Rack loader. Reagent compartment. Processed tubes. Technical software. Stat sample Bay. Validation station.

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V 2.01

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  1. V 2.01 In the heart of hematopoiesis

  2. ABX PENTRA DX 120 • Main features analysis • Measurement principles • Expert Validation Station • SPS Evolution

  3. External view Rack loader Reagent compartment Processed tubes Technical software Stat sample Bay Validation station Optional slide maker and stainer

  4. Main features • CBC, DIFF, Reticulocytes, NRBC’s • IMmature Lymphocyte, Monocyte and Granulocyte • Optional and integrated slide maker and stainer • Expert Validation Station

  5. Main features 45 Parameters • CBC (12 parameters) • Wbc, Rbc, Hgb, Hct, Mcv, Mch, Mchc, Rdw, Plt, Mpv, Pct, Pdw • Differential count (12+20 parameters) • Lym, Mon, Neu, Eos, Baso, Aly, Lic (% and #) • IML, IMM, IMG (% and #) • Reticulocyte count (10 parameters) • Ret (% and #), Retl%, Retm%, Reth%, Imm%; Crc%, Mrv, Mfi%, Irf • ERB (3 parameters) • ERB (% and #), Cwbc (Corrected WBC)

  6. Main features • 6 reagents • ABX Diluent • ABX Basolyse • ABX Leucodiff • ABX Alphalyse or ABX Lysebio (Cyanide free) • ABX Fluocyte (1L or 1/2 L) • ABX Cleaner • 3 controls • ABX Difftrol • ABX Minotrol Retic • ABX Erytrol ABX reagents and controls are bar-coded

  7. Main features • 8 types of analyses (with random access) • CBC • DIFF • RET • CBR • DIR • ERB • CBE • SPS External and internal bar-code reader

  8. Measurement principles • Main features analysis • Measurement principles • Expert Validation Station • SPS Evolution • CBC principles • 5 Diff –Double matrix • Reticulocyte • NRBC’s (ERB)

  9. Measurement principles CBC measurement principles

  10. Measurement principles RBC/PLT - Impedance method HGB - Cyanmethemoglobin or cyanide free BASO - Specific lyse & impedance method WBC - (Quadimension) -> WBC channel (impedance) -> BASO channel (impedance) -> 5Diff channel (optical) -> ERB channel (fluorescence)

  11. Measurement principles Double DIFF matriX

  12. Measurement principles 5Diff Principles  • 1. Cytochemistry • Lysing of red blood cells • Staining of nuclei and cytoplasmic granules of leukocytes • Fixing the leukocytes in their original state (48 hours post-draw stability) • 2. Double Hydrodynamic Sequential System (DHSS) • Flow-cytometer with hydrodynamic focusing before and after the micro-aperture • 3. Impedance method • Measurement of the cell volume (“X” axis)    • 4. Light absorbance • Internal structure of cells (“Y” axis)

  13. Measurement principles Eosinophils Large Immature Cells Neutrophils Lymphocytes Monocytes Atypical LYmphocytes Double Diff Matrix IMmature Granulocytic line IMmature Monocytic line IMmature Lymphocytic line

  14. Measurement principles Metamyelocyte Myelocyte Promyelocyte Myeloblast IMmature Granulocytes Granulocytic line IMG

  15. Measurement principles Promonocyte Monoblast IMmature Monocyte Monocytic line IMM

  16. Measurement principles IMmature Lymphocyte Lymphocytic line Lymphoblast Prolymphocyte IML

  17. Measurement principles • Routine identification and quantification of 3 immature cell lines involved in leucopoiesis: • Granulocytic line • Monocytic line • Lymphocytic line -> Reduction of the number of slide examinations -> Diagnostic and follow-up tool for rapid decision-making

  18. Measurement principles Ne: (Neutro-eosino) Hyper-segmented neutrophils Hypo-granular eosinophils... ALY: (Atypical Lymphocytes) Atypical Lymphocytes Plasmocytes, hairy Cells... LIC: (Large Immature Cells) Blasts, myelocytes Promyelocytes Metamyelocytes... Mn: (Mono-neutro) Immature granulocytes Band cells... Nl: (Neutro-lympho) Hypo-segmented neutrophils Sezary cells, hairy cells... 1- Priority alarms 2- Separation alarms

  19. Measurement principles No: (Noise) Platelet aggregates, NRBCs Lysis resistant RBCs Ln: (Left-neutro) Cellular debris Ll, Ll1: (Left-lympho) Platelet aggregates, NRBCs Rn: (Right-neutro) Immature granulocytes Rm: (Right-mono) Large monocytes, blasts 3- Zone alarms

  20. Measurement principles Reticulocytes

  21. Measurement principles   Reticulocyte count  • 1. Fluorochrome • Reticulocytes are stained according to the RNA they contain. • 2. Double Hydrodynamic Sequential System (DHSS) • Flow-cytometer using hydrodynamic focusing before and after the aperture • 3. Impedance method • Measurement of the cell volume (“X” axis)  • 4. Fluorescence (and forward scatter) • RNA content (“Y” axis)

  22. Measurement principles RET Fluorescence RBC Volume Diagnostic of anemia 3 maturation stages according to the RNA content in reticulocytes : RET High, RET Medium, RET Low IRF -> Immature Reticulocyte Fraction MFI -> Mean Fluorescence Index Sensitive indicators to the bone marrow response CRC -> Corrected Reticulocyte Count Diagnosis of generative or non-generative anemia MRV -> Mean Reticulocyte Volume Erythropoietic regeneration in iron deficient anaemias

  23. Measurement principles NRBC’s

  24. Measurement principles   NRBC’s count (ERB)  • 1. Specific lyse and fluorochrome • a) Lysing of RBC • b) Staining of WBC and NRBC’s according to the DNA they content. • 2. Double Hydrodynamic Sequential System (DHSS) • Flow-cytometer using hydrodynamic focusing before and after the aperture • 3. Impedance method • Measurement of the cell volume (“X” axis)  • 4. Fluorescence (and forward scatter) • DNA content (“Y” axis)

  25. Measurement principles NRBC (ERB) principles WBC ERB Fluorescence Volume Leukocyte count is automatically corrected in case of erythroblast presence.

  26. Measurement principles               5 recognised measurement principles

  27. Expert validation station • Main features analysis • Measurement principles • Expert Validation Station • SPS Evolution • Specs & functionality

  28. Specs & functionality Dedicated PC 17 inch flat colour screen Windows XP CD-ROM burner Automatic rerun Reflex testing Pre-diluted mode Cytology library Onboard user manual

  29. Specs & functionality • Mono or bi-directional connection: • ASTM format • Transfer of • Demographic data (blood type - man, woman, child) • Tests requested (CBC, CBC/DIFF, RET...) • Connection of 3 analyzers • Query mode • Download capability

  30. Specs & functionality New D+ D- Old Delta check User defined rules _ Rerun capability _ Reflex testing _ Automatic validation _ Messages, comments…. • Bio-technical validation with : • Patient history, delta check • Result edition • Manual entry • Recalculation capability

  31. Specs & functionality 20 000 results 1 matrix 3 histograms Zoom capability

  32. Specs & functionality Hematovision IV included A cell cytology library

  33. SPS EVOLUTION • Main features analysis • Measurement principles • Expert Validation Station • SPS Evolution • Specification • Technology

  34. Specification Throughput : 120 stained slides per hour Sample volume: 50µl Stain : MGG, Wright, Wright-Giemsa User defined smear criteria Smearing profile function to each individual sample and to each hematology parameter Automatic maintenance

  35. Technology Slide stacker capacity of160 slides When dispensed, slides are twisted to avoid blockages Rack stacker capacity of8 racks Located outside, slides and racks are easily reloaded at any time

  36. Technology Titanium coated wedge : No change, no adjustment Smearing tape : Moves forward between each sample to avoid contamination and to avoid a complex mechanism to clean and dry the wedge

  37. Technology User’s defined smearing profile : - Blooddrop volumeadjustable by increment of 0,1 µl - 2 smearing angles (40° or 45°) - Waiting time adjustable by increment of 1ms - Smearing speed adjustable by increment of 1step/ms

  38. Technology

  39. Technology Dryingprocess : Slide identification : 3 lines of 14 alpha-numerical characters

  40. Technology Individual staining wells: Use of fresh stains ensures a good staining stability User defined stain volume Automatic stain dilution Staining steps adjustable by increment of 30s Maximum staining duration of 16mn

  41. Technology Manual mode : Pre-smeared slide: Bone marrow Biologic fluid Cyto-centrifuging Leuko-concentration Slide outlet : 20 slides can collected in one rack

  42. Technology Manual smear SPS Evolution • Manual identification • Irregular blood films • Irregular staining • Automatic identification • Homogenous blood films • Standardization of the staining

  43. ABX pentra 120 series

  44. ABX Pentra 120 series

  45. In the heart of hematopoiesis

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