How can trees be so tall...and get water to their tops?

1. How can trees be so tall...and get water to their tops?

2. Also, check out the Bioflix on water transport

3. Transport in Plants

4. Transport in plants • H2O & minerals • transport in xylem • transpiration • evaporation, adhesion & cohesion • negative pressure • Sugars • transport in phloem • bulk flow • Calvin cycle in leaves loads sucrose into phloem • positive pressure • Gas exchange • photosynthesis • CO2 in; O2 out • stomates • respiration • O2 in; CO2 out • roots exchange gases within air spaces in soil Why doesover-wateringkill a plant?

5. Ascent of xylem fluid Transpiration pull generated by leaf

6. Water & mineral absorption • Water absorption from soil • osmosis • aquaporins • Mineral absorption • active transport • proton pumps • active transport of H+ aquaporin root hair proton pumps H2O

7. Mineral absorption • Proton pumps • active transport of H+ ions out of cell • chemiosmosis • H+ gradient • creates membranepotential • difference in charge • drives cation uptake • creates gradient • cotransport of othersolutes against theirgradient

8. Water flow through root • Porous cell wall • water can flow through cell wall route & not enter cells • plant needs to force water into cells Casparian strip

9. Controlling the route of water in root • Endodermis • cell layer surrounding vascular cylinder of root • lined with impermeable Casparian strip • forces fluid through selective cell membrane • filtered & forced into xylem cells Aaaah… Structure–Functionyet again!

10. Root anatomy dicot monocot

11. Mycorrhizae increase absorption • Symbiotic relationship between fungi & plant • symbiotic fungi greatly increases surface area for absorption of water & minerals • increases volume of soil reached by plant • increases transport to host plant

12. Mycorrhizae

13. Transport of sugars in phloem • Loading of sucrose into phloem • flow through cells via plasmodesmata • proton pumps • cotransport of sucrose into cells down proton gradient

14. Pressure flow in phloem • Mass flow hypothesis • “source to sink” flow • direction of transport in phloem is dependent on plant’s needs • phloem loading • active transport of sucrose into phloem • increased sucrose concentration decreases H2O potential • water flows in from xylem cells • increase in pressure due to increase in H2O causes flow can flow 1m/hr On a plant…What’s a source…What’s a sink?

15. Experimentation • Testing pressure flow hypothesis • using aphids to measure sap flow & sugar concentration along plant stem

16. Maple sugaring

17. Any Questions?

18. A step further

19. Endodermis & Casparian strip

20. Control of Stomates Epidermal cell Nucleus Guard cell Chloroplasts • Uptake of K+ ions by guard cells • proton pumps • water enters by osmosis • guard cells become turgid • Loss of K+ ions by guard cells • water leaves by osmosis • guard cells become flaccid K+ K+ H2O H2O H2O H2O K+ K+ K+ K+ H2O H2O H2O H2O K+ K+ Thickened inner cell wall (rigid) H2O H2O H2O H2O K+ K+ K+ K+ Stoma open Stoma closed water moves into guard cells water moves out of guard cells

21. Control of transpiration • Balancing stomate function • always a compromise between photosynthesis & transpiration • leaf may transpire more than its weight in water in a day…this loss must be balanced with plant’s need for CO2 for photosynthesis

22. REVIEW • How does transport differ going up versus going down in plants? • Why do roots look like roots? • List three different plant functions requiring symbiotic partners.