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Project 1. An in vitro fast, convenient and non-radioactive assay for quantitative measurements of enzymatic activity is developed. This assay will facilitate the generation of kinetic data for feeding the hybrid model.
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Project 1 An in vitro fast, convenient and non-radioactive assay for quantitative measurements of enzymatic activity is developed. This assay will facilitate the generation of kinetic data for feeding the hybrid model These hybrid models will include the discrete states of the Base Excision repair process along with the underlying continuous-time dynamics that describe the fundamental biochemical reactions between the enzymes and substrates. A GFP based in vivo BER assay is developed to validate the model under native conditions A mathematical model of in vitro reconstituted Base Excision repair as operative on IUdR generated mispairs