Genikon

1. Genikon The New 3D Generation of in Situ Fluorescence Hybridisation The Motorization of the 90i Nikon Microscope Nikon Software interface for the control of the microscope Dapi + Fitc + Tritc = Epi_fluorescence turret motorization Fish Analysis on lymphocyte, chromosome 4 (rearranged photo b (Dr.Antonio Novelli, Roma) The new digital head of the microscope 90i/80i gives the opportunity to be controlled from software in the moving of the single filter in a fast a very easy way. This opportunity eliminates shifting and bleaching problems. The software offers list of capturing saved and the complete control of the shutter of the fluorescence in order to illuminate as less as possible the sample. The evolution of the microscopes during last years ad their Automation (z axe control, control of filter set ) gave a big contribution to the precision of the acquisition of images in FISH technique. The fusion of Nikon microscopes with Genikon “Digital System” gives today the ideal solution to acquire and to process FISH images in a very quick and simple way. In particular, the automatic control of the times acquisition of the cameras, gives possible a completely automatic solution. Building of the fluorochromes list by the set up of the number of planes of focus Microscope’s filters control by Genikon interface With just a click of the mouse the user starts the acquisition process of all the fluorochromes of the list and gives to the system the complete control of the exposition times in order to obtain at the end a perfect and focussed image.

2. Autofocus control Tool Senza “Without Autofocus tool” “With Autofocus tool” The new tool of automatic focus control, identifies the best contrast for every plane for each fluorochrome independently from the one fixed at the ocular Set up of Acquisition sequence on every plane of focus Fusion Image of every focus planes Single plane image Because in a single plane of fluorescence could happen that one signal doesn’t show the same focus of the other, the new tool of 3D reconstruction of Genikon became very important. This function will create one single image which will show all the single signals (for the same fluorochrome) at the same focus plane. Thanks to the new techniques of 3 D reconstruction and of focus control, it’s possible to execute a building of Karyotyping also on Fish samples Karyotyping on Fish images Genikon with the 3D for automatic method of counting spot, offers a new procedure fast and precise to obtain the value of the ratio of spots on single nuclei or many nuclei identified from the user on HER2NEU samples. Funzione “Dapi reverse” The DAPI reverse function it’s showing the probe and helps the user to recognise chromosomes HER2/Neu Analysis NIKON INSTRUMENTS S.p.A. Firenze (Calenzano) Via Meucci N.59 Tel.055 3009601 - Fax 055 300995 www.nikon.it e-mail:info@nikon.it