1 / 20

Rotation Automation Motivation

Rotation Automation Motivation. How the Liverpool Lab works. What we aim to do. Meet our service needs Meet our turnaround times Keep staff happy!. How have we achieved this. Increased use of automation Integration of database and instruments Changing Rotation system. GT Rotation.

patsy
Download Presentation

Rotation Automation Motivation

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. RotationAutomationMotivation How the Liverpool Lab works

  2. What we aim to do • Meet our service needs • Meet our turnaround times • Keep staff happy!

  3. How have we achieved this • Increased use of automation • Integration of database and instruments • Changing Rotation system

  4. GT Rotation • Disorders split into 4 groups • Group 1 Triplet Repeats • Group 2 Single gene disorders • Group 3 Sequencing • Group 4 Aneuploidy Screen, Telomeres

  5. Current Rotation System • 4 months on each group Working with a lead scientist • 1 month rotation into DNA extraction

  6. This talk concentrates on areas of recently increased automation • DNA Extraction • Sequencing service

  7. DNA Extraction • DNA extracted from most blood samples by Chemagen/Perkin Elmer DNA Extraction system. • Small blood samples and all other tissues by Qiagen EZ1.

  8. Automation of DNA Extraction • Barcode reader for blood tubes and elution tubes • High risk protocol recently introduced • Encourage (some) use of Oragene kits so DNA can be processed by Chemagen to improve DNA quality.

  9. DNA Extraction data • Use Nanodrop to generate quantity and quality information on each sample. • Import this data into database

  10. Sequencing service • GT responsible for generating PCR products and sequencing. • Co-ordinating PCR products submitted by other staff.

  11. Submitting PCR products for sequencing • Details of Gene, Exon, primers etc put onto database by individual staff • This information used by GT to create plate record for Biomek and ABI 3730

  12. Pre and post PCR Biomek robots • PCR set up • Post PCR • Ampure (PCR clean up) • Sequencing set up • Cleanseq (sequencing clean up) • Elution into plate for ABI 3730

  13. Recent developments • Increased use of pre PCR robot to set up DNA dilutions and PCR reactions • Working towards the use of StarLims

  14. Summary • GTs rotate through all sections of the lab in around 18 months. • This helps with continuity of service during sickness and holidays • Increasing automation and integration avoids repeating tasks

More Related