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Jan-Willem de Gier Center for Biomembrane Research, Stockholm, Sweden

Green Light for Membrane Proteins. Jan-Willem de Gier Center for Biomembrane Research, Stockholm, Sweden. David Drew et al. Nat Methods (2006) 4:303-13. Monitoring overexpression of a YedZ-GFP fusion using in-gel and whole-cell fluorescence. David Drew et al. Nat Methods (2006) 4:303-13.

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Jan-Willem de Gier Center for Biomembrane Research, Stockholm, Sweden

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  1. Green Light for Membrane Proteins Jan-Willem de Gier Center for Biomembrane Research, Stockholm, Sweden

  2. David Drew et al. Nat Methods (2006) 4:303-13

  3. Monitoring overexpression of a YedZ-GFP fusion using in-gel and whole-cell fluorescence David Drew et al. Nat Methods (2006) 4:303-13

  4. From detergent screen to recovered membrane protein David Drew et al. Nat Methods (2006) 4:303-13

  5. Recovered membrane proteins can be used for functional and structural studies David Drew et al. Protein Sci (2005) 14: 2011-2017

  6. GFP-based pipeline in yeast Simon Newstead et al. PNAS (2007) 104(35):13936-41

  7. Biogenesis of membrane proteins in E. coli membrane proteins secretory proteins Samuel Wagner et al. (2006) Trends Biotechnol 24: 364-371

  8. E. coli BL21(DE3)/pET overexpression system Merck Novagen, pET manual

  9. Experimental setup (1) Samuel Wagner et al. (2007) Mol Cell Proteomics (9) 1527-50.

  10. Experimental setup (2) Samuel Wagner et al. (2007) Mol Cell Proteomics (9) 1527-50.

  11. Consequences of membrane protein overexpression in E. coli (1) Samuel Wagner et al. (2007) Mol Cell Proteomics (9) 1527-50.

  12. Consequences of membrane protein overexpression in E. coli (2) Samuel Wagner et al. (2007) Mol Cell Proteomics (9) 1527-50.

  13. Characterization of C41(DE3) and C43(DE3), also known as the Walker strains

  14. Differences in overexpression kinetics? Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.

  15. Differences in overexpression kinetics Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.

  16. Effect of the addition of IPTG Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.

  17. Mutations in the promoter governing T7RNAP expression in C41(DE3) and C43(DE3) Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.

  18. Mimicking the effect of the mutations in the promoters with T7 lysozyme Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.

  19. Mimicking the effect of the mutations in the promoters with T7 lysozyme Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.

  20. Characterization of Lemo21(DE3) Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.

  21. Lemo21(DE3) is an ’all in one’ platform for membrane protein overexpression Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.

  22. www.cbr.su.se Funding -Swedish Research Council (VR) -Wallenberg Foundation -Swedish Foundation for Strategic Research (SSF) -National Institutes of Health (NIH) www.xbranebio.com

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