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Explore Accellix's innovative delivery platform for precise on-demand analytics of microfluidic cartridges, bead assays, cell assays, and automated data analysis for immunotherapy. Enhance your research workflow efficiently!
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ON DEMAND ANALYTICS Accellix Delivery Platform Ed Farnam September. 2018
ON DEMAND ANALYTICS Accellix Delivery Platform
ON DEMAND ANALYTICS Accellix Delivery Platform
ON DEMAND ANALYTICS Accellix Delivery Platform
ON DEMAND ANALYTICS Accellix Delivery Platform Microfluidic Cartridge Dry Reagents Measurement System Automated Data Analysis Immunotherapy • Bead Assays • Cell Assays • Enzymatic • PCR
ON DEMAND ANALYTICS Accellix Delivery Platform
ON DEMAND ANALYTICS Accellix Laser & 11 Detectors 488526 567 606 643 678 712 743 772 800 826 852
ON DEMAND ANALYTICS Accellix Detectors & Fluorophores • 628–658 590–622 550–584 698–725 664–692 730–755 508–544 841–862 788–811 760–784 815–837 Example Fluorophores: FITC PE PE-TexasRed PE-Cy5 PE-AF700 PE-Cy7
ON DEMAND ANALYTICS Accellix Simplified Workflow Add 40uL Sample to Reagent Tube Add 20uL Sample mixture to Cartridge inlet port Mix sample with unitized dry reagent Load Cartridge into Instrument
ON DEMAND ANALYTICS Accellix T Cell Panel A488 PE PE-D594 V631 PE-CY5 Alexa 488 anti CD45 PE anti-CD4 PE-D594 anti-CD8 V631 dead cell stain PE-Cy5 anti CD3
ON DEMAND ANALYTICS Accellix T Cell Panel Gate CD45 Gate CD3 Forward Scatter D594 Forward Scatter Alexa 488 PE Cy5 PE
ON DEMAND ANALYTICS Accellix Stem Cell Panel A488 PE 7-AAD Alexa 488 anti CD45 PE anti-CD34 7-AAD dead cell stain Developed using ISHAGE reagent formulation ofantiCD45-FITC, antiCD34-PE, 7-AAD, and counting beads.
ON DEMAND ANALYTICS Accellix Stem Cell Panel Gate CD45 Counting Bead determination from all events CD45+ determinationfrom all events CD34+ determinationfrom CD45+ cells Dead cell determinationfrom CD45+ Cells
ON DEMAND ANALYTICS Accellix Beads and Cytokines Beads withCapture Antibodies Cytokines Excite withLaser andread in FlowCell Labelled Antibodies Images: bdbiosciences
ON DEMAND ANALYTICS Accellix Beads and Cytokines
ON DEMAND ANALYTICS GSH Mito All Nucleated Cells Accellix Cell Health Panel Annexin V Viability Glutathione (GSH) Oxidative Stress Annexin V Later Apoptosis Mitochondrial Membrane Potential Early Apoptosis Dead Cells Membrane integrity DNA stain All Nucleated Cells
ON DEMAND ANALYTICS Accellix Cell Health Panel All Nucleated Cells identification: DNA stain for all live and dead cells. Gate out debris for other dyes.
ON DEMAND ANALYTICS Accellix Cell Health Panel GSH-Glutathione: Assess capacity for RedOx regulation (Oxidative Stress).
ON DEMAND ANALYTICS GSH-Glutathione: 0-6 hours post Menadione
ON DEMAND ANALYTICS Mitochondrial Membrane Potential: 0-6 hours post Menadione
ON DEMAND ANALYTICS Annexin V binding: 0-6 hours post Menadione
ON DEMAND ANALYTICS Viability (dead cell) stain: 0-6 hours post Menadione
ON DEMAND ANALYTICS Marker Status
