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Real World Project: Determination of Vitamin C in Fruit Juices. Brian Gillease And Lauren Burns. Objective. Calculate vitamin C in a Vitamin C tablet Titrate fruit juices Calculate amount of vitamin C in each juice Compare juices to tablet. Importance. Vitamin C –
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Real World Project: Determination of Vitamin C in Fruit Juices Brian Gillease And Lauren Burns
Objective • Calculate vitamin C in a Vitamin C tablet • Titrate fruit juices • Calculate amount of vitamin C in each juice • Compare juices to tablet
Importance • Vitamin C – • antioxidant; binds with carbon, hydrogen, oxygen; oxidizes easily • Safe and efficient nutrient, helps protect body against cardiovascular disease, eye disease, and other diseases
Procedures • Standardized thiosulfate solution using a starch paste • Titrated a vitamin C tablet with thiosulfate to determine the amount of ascorbic acid present • Juiced fruits • Centrifuged fruits • Titrated with thiosulfate
Procedures - Errors • Juicer • too much pulp • Filtration • pulp too thick • Strawberry Juice • red color of juice
Error Source • Over titrated thiosulfate • Not enough KI • Wasn’t an excess of KI to react with • Refrigerate • Juices were not refrigerated • Solutions degrade • KIO3 degraded; did not work
Conclusion • Papaya juice contained the most Vitamin C; aligned with FDA statistics • Numerous errors throughout • Overall experiment was unsuccessful; several areas that could be improved