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Ardrossan Academy Dead Earth

Learn how to test a leaf for starch to determine photosynthesis activity. Understand conditions required for photosynthesis and the importance of chlorophyll, light, and carbon dioxide for plant growth. Follow a step-by-step method and observe iodine test results.

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Ardrossan Academy Dead Earth

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  1. Ardrossan AcademyDead Earth Subtopic – Photosynthesis contd.

  2. Lesson Title: Testing a Leaf for Starch

  3. Photosynthesis recap animation • http://www.kscience.co.uk/animations/photosynthesis.swf

  4. Photosynthesis Photosynthesis

  5. GLUCOSE is produced during photosynthesis and stored as STARCH • If a leaf has starch in it, it means photosynthesis has been taking place • IODINE solution turns blue/black if starch is present

  6. Testing a leaf for starch Testing a leaf for starch

  7. Method - 1. Boil the leaf in water for 5 min (to soften the leaf) 2. PUT OUT THE BUNSEN BURNER 3. Use the glass rod to transfer leaf into boiling tube 4. Place boiling tube back into the hot water to boil the leaf in alcohol (to remove the green colouring) 5. Carefully lift the leaf out with glass rod and place in hot water to wash the leaf 6. Spread the leaf out on a dimple tile. 7. Test with Iodine solution Animation 1. Boil in water - (water in beaker) 2. Boil in alcohol 3. Rinse alcohol off leaf 4. Test leaf with Iodine solution - (Bunsen switched off !! )

  8. IF the iodine turnsblue/blackTHENthe leaf has starch. Starch present Starch absent In the presence of light green plants are able to make starch (food) in their leaves

  9. Aim • To investigate the conditions required for photosynthesis What conditions do you think are needed for photosynthesis?

  10. Method - 1. Boil the leaf in water for 5 min (to soften the leaf) 2. PUT OUT THE BUNSEN BURNER 3. Use the glass rod to transfer leaf into boiling tube 4. Place boiling tube back into the hot water to boil the leaf in alcohol (to remove the green colouring) 5. Carefully lift the leaf out with glass rod and place in hot water to wash the leaf 6. Spread the leaf out on a dimple tile. 7. Test with Iodine solution Animation 1. Boil in water - (water in beaker) 2. Boil in alcohol 3. Rinse alcohol off leaf 4. Test leaf with Iodine solution - (Bunsen switched off !! )

  11. Green Green white Green Green

  12. Conclusion • I found out that . . . . (refer back to your aim) • Carbon dioxide, light and chlorophyll are needed for photosynthesis to take place.

  13. Lesson 2 - Learning Intentions • Through practical work, name the conditions required for photosynthesis to take place

  14. POSTER • Title – ‘Do plants need chlorophyll / light / carbon dioxide for photosynthesis’ • Method, including diagrams, of how you carried out the experiment • Results – what colour did the iodine test produce • Conclusion, what do plants need for photosynthesis. • Word Equation

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