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Cytokine Expression in the RUPP Rat Model

Background. What is preeclampsia?Major symptoms include hypertension, edema, and protein in the urineDiagnostic issues include symptomatic diagnosisPossible causes are mostly related to hypertension. . Introduction. Preeclampsia is responsible for 10% of pregnancy related deaths annuallyPrecla

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Cytokine Expression in the RUPP Rat Model

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    1. Cytokine Expression in the RUPP Rat Model Michelle Hatch Kedra Wallace, Mentor

    2. Background What is preeclampsia? Major symptoms include hypertension, edema, and protein in the urine Diagnostic issues include symptomatic diagnosis Possible causes are mostly related to hypertension

    3. Introduction Preeclampsia is responsible for 10% of pregnancy related deaths annually Preclampsia is a leading cause of premature birth, infant death, and other developmental problems The role of inflammatory cytokines in preeclampsia

    4. Hypothesis Inflammatory cytokine levels will be increased in the RUPP brain tissue in comparison to the control.

    5. Methods Immunohistochemical methods Rat brains with skull intact were taken at the 18th day of gestation from both control and RUPP treated adult female rats and were snap frozen. Sections were taken at 50 microns with a cryostat and fixed to slides. Tissues were stained for IL-1B, IL-10, and TNF-a proteins. Tissues were examined by microscope to determine the presence of positive staining for each protein and the region of staining.

    6. Rupp Model Reduced Uterine Placental Perfusion model Small silver clip is placed on the bifurication of the uterine horn. Clip creates a reduced oxygen environment which creates characteristics similar to preeclampsia.

    7. Results

    8. Cytokine expression for Tumor Necrosis Factor-alpha was decreased in the RUPP model at day 18 of gestation.

    9. Cytokine expression for Interleukin 1� was decreased in the RUPP model at day 18 of gestation.

    10. Cytokine expression of Interleukin 10 was decreased in the RUPP model at day 18 of gestation.

    11. Conclusion There is a decrease in cytokine levels at gestation day 18 for the RUPP model. Role of cytokine producing astrocytes Unknown factors Small sample size

    12. Future possibilities Test for GFAP to determine the level of astrocytes.

    13. A Special Thanks To: Kedra Wallace Dr. William Bennett Dr. James Shaffery Dr. Joey Granger Dr. Ian Paul Center for Psychiatric Neuroscience Neuroscience Scholars Program

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