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What to feed yellowtail kingfish cultured in cold water?. JENNA BOWYER PhD Supervisors A/Prof. Jian Qin Flinders University Dr. David Stone SARDI Aquatic Sciences (SUSTAINABLE FEEDS & FEED MANAGEMENT FOR YELLOWTAIL KINGFISH). Introduction. Y ellowtail kingfish, Seriola lalandi (YTK).
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What to feed yellowtail kingfish cultured in cold water? JENNA BOWYER PhD Supervisors A/Prof. Jian Qin Flinders University Dr. David Stone SARDI Aquatic Sciences (SUSTAINABLE FEEDS & FEED MANAGEMENT FOR YELLOWTAIL KINGFISH)
Introduction Yellowtail kingfish, Seriola lalandi (YTK) Clean Seas Tuna (CST) currently produces 5000 Mt yr-1
CST YTK aquaculture sites in SA Port Augusta Arno Bay SPENCER GULF Port Lincoln CST plan to boost production to 10,000 Mt yr-1
YTK are grown at fluctuating water temps in Spencer Gulf (11-24°C) Water temp (°C)
Production cycle of YTK grown at fluctuating water temps in Spencer Gulf (11-24°C) stocking harvest Water temp (°C) Harvest at 3 - 4kg (1 winter) Stock fish at 5 g (Oct/Nov) Miss harvest window = loss of productivity 15-18 month production cycle
Effects of water temperature on growth YTK are grown at fluctuating water temps in Spencer Gulf (11-24°C) Best growth Moderate growth (Shoulder periods) Water temp (°C) Low or no growth
To improve the condition coming into winter (sub-optimal temperatures, 17-19°C) in an attempt to get better growth coming out of winter to meet harvest schedule Aim Shoulder period Shoulder period Water temp (°C) Need better understanding of the nutritional requirements of YTK cultured at 17-19°C
Objectives • Explore baseline growth performance, condition and feed efficiencies for YTK cultured at different water temperatures ranging from 12 to 24°C • Determine effective protein: energy ratios for YTK at optimal (24°C) & sub-optimal temperatures (18°C) • Replace fish meal & fish oil with sustainable alternative plant & animal protein & lipid sources at optimal (24°C) & sub-optimal temperatures (18°C)
Experimental facilities • South Australian Research & Development Institute – Aquatic Sciences • Temperature controlled recirculating & flow-through systems • Nutrition laboratory • 24 × 700-L fibreglass tanks • Aquarium room • 36 × 170-L fibreglass tanks
Experimental facilities • Department of Primary Industries, NSW Port Stephens Fisheries Institute • Temperature controlled recirculating & flow through systems Source: Dr. Mark Booth, PSFI, NSW
Experiment 1: Growth performance, condition & feed efficiencies of YTK at 12 to 24°C Aim: Benchmark growth performance of YTK • Location: NSW DPI, Port Stephens Fisheries Institute (PSFI) • Diet : Reference diet (45% protein, 20% lipid) • Fish size: ~500g YTK • Replication: 3 tanks/treatment • Duration: 8 weeks
Experiment 2a & 2b: Optimum protein: energy ratios for small & large YTK at 18 & 24°C Aim: Determine optimum P:E ratios for YTK • Experiment 2b • ~250g (initial) YTK • 2 tanks/ treatment • 8 weeks • Experiment 2a • ~5g (initial) YTK • 3 tanks/ treatment • 4 weeks • Location: SARDI Aquatic Sciences, West Beach SA • Diets: formulated using fish meal & fish oil as the primary ingredients
Experiments 3 & 4: Evaluation of alternative protein sources at 18°C & 24°C Aim: Investigate the suitability of sustainable alternative protein sources to replace fish meal for YTK • Location: SARDI Aquatic Sciences, West Beach SA • Test products: soybean, canola, lupin & poultry (TBD) • Diets: 5 diets to be formulated to contain equal amounts of protein, lipid & energy (TBD) & balanced for lysine & methionine • Fish size: ~5g (initial) • Replication: 3 tanks/treatment • Duration: 4 weeks
Experiments 5: Alternative lipid sources (canola oil, poultry fat) at 18°C & 24°C Aim: Investigate the growth performance of YTK when fed sustainable alternative lipid sources to replace fish oil • Location: SARDI Aquatic Sciences, West Beach SA • Test products: canola oil & poultry fat (TBD) • Diets: test products to replace fish oil in dietary formulation • Fish size: small fish ~5g • Replication: 3 tanks/treatment • Duration: 4 weeks
Sample analysis • Growth performance/ condition/ feed efficiency • Specific growth rate (%BW/ day), daily feed intake, feed conversion ratio, condition index, protein & energy retention efficiencies, fillet yield, hepatosomatic index • Apparent digestibility coefficient (ADC) • Run after completion of growth trial • Faeces will be collected by manual stripping • Proximate composition of faeces and diets • Fillet fatty acid composition • product quality • Proximate composition • Nutrient retention efficiencies & product quality • Lipid oxidation (liver) • Thiobarbituric acid reactive substances (TBARS) • Vitamin E
Rainbow trout, (Oncorhynchus mykiss) digestive tract Sample analysis • Gut bacterial flora • Identify the bacterial communities in the anterior & posterior gut sections, using Terminal Restriction Fragment Length Polymorphism (TRFLP) • Histology • Identify any physical changes in sections of the anterior & posterior digestive tract • Enzyme analysis • Determine the enzymatic activity of pepsin, trypsin, chymotrypsin, lipase & amylase from sections of the digestive tract • Plasma • Cholesterol • Triacylglycerides Normal villi of large intestine, longitudinal section of outer muscular layer, a thin pink-staining stratum compactum & villi. Villi are long, have space between them. Apical vacuoles seen in mucosal epithelium. Fusion of intestinal villi. Villi are somewhat shortened adjacent to the fused ones.
Outcomes • Directly benefit YTK industry in Australia • By providing information to develop sustainable, cost effective dietary formulations, i.e. • Identification of optimum P:E ratios • Replacement of fish meal & fish oil with more sustainable plant & terrestrial sources to reduce ingredient costs • Leading to enhanced sustainable productivity of YTK cultured at sub-optimal water temperatures
“ This work formed part of a project of the Australian Seafood Cooperative Research Centre, and received funds from the Australian Government’s CRCs Programme, the Fisheries R&D Corporation and other CRC Participants”. Acknowledgements • Australian Seafood CRC • Clean Seas Tuna Ltd. • Mr. Mike Thomson • Mr. Chester Wilkes • Flinders University • Dr. Kathy Schuller • SARDI-Aquatic Sciences • Dr Valeria Torok • Department of Primary Industries Port Stephens • Dr. Mark Booth • University of Tasmania • Dr. Louise Ward