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Chapter Six – Separation Methods

Chapter Six – Separation Methods. FRSC 8104 Criminalistics II Professor Bensley. FRSC 8104 – Chapter 6 Objectives. After studying this chapter, the student should be able to:  Describe solid and liquid phase extractions and recognize when these techniques are useful

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Chapter Six – Separation Methods

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  1. Chapter Six – Separation Methods FRSC 8104 Criminalistics II Professor Bensley

  2. FRSC 8104 – Chapter 6 Objectives After studying this chapter, the student should be able to:  Describe solid and liquid phase extractions and recognize when these techniques are useful  Understand the basic concepts behind chromatographic methods Recall the important components of a gas chromatograph and a high performance liquid chromatograph and their function  Recognize how chromatographic techniques are employed in the forensic laboratory; know which separation techniques are useful for the various types of evidence encountered in the laboratory

  3. FRSC 8104 – Chapter 6 Outline • Introduction • Why are these techniques needed? • Definitions: Analyte, Solvent, Solute • Liquid Phase Extraction • Partitioning Process • Polarity • pH

  4. FRSC 8104 – Chapter 6 Outline

  5. FRSC 8104 – Chapter 6 Outline • Solid Phase Extraction • Adsorption • Examples

  6. FRSC 8104 – Chapter 6 Outline • Chromatography • History and Theory • Stationary vs. Mobile Phases • Normal and Reverse Phases • Gas Chromatography (GC or GLC) • Packed vs. Capillary Columns • Components • Injection Port • Column • Detector • Quantitative Analysis • Pyrolysis GC • Applications

  7. FRSC 8104 – Chapter 6 Outline • High Performance Liquid Chromatography (HPLC) • Advantages over GC • Components • Injector • Column • Detector • Applications

  8. FRSC 8104 – Chapter 6 Outline • Thin Layer Chromatography (TLC) • Stationary vs. Mobile Phases • Process • Detection • Applications • Advantages/ Disadvantages

  9. FRSC 8104 – Chapter 6 Outline • Electrophoresis • Gel vs. Capillary (CE) • Stationary Phases • Mobile Phase • Detectors • Applications Animation Detailing Capillary Electrophoresis

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