1 / 18

Odile Huchette, research engineer Christèle Bellamy, research technician

G&H October 2003, Münich WP3 / cultivation, P4 P4 : persons involved. Odile Huchette, research engineer Christèle Bellamy, research technician Didier Perrin and Eric Vade, research assistants.

phyllis-filia
Download Presentation

Odile Huchette, research engineer Christèle Bellamy, research technician

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. G&H October 2003, MünichWP3 / cultivation, P4 P4 : persons involved • Odile Huchette, research engineer • Christèle Bellamy, research technician • Didier Perrin and Eric Vade, research assistants Influence of environmental conditions, genetic factors and mineral nutrition on the alliin content of garlic bulbs COOPD'OR R&D

  2. G&H October 2003, Münich WP3 / cultivation, P4 Summary of the main conclusion of the experiments 2000-2002 • Very high differences of alliin contents were observed in field trials between France and Spain : the soil as a factor of explanation but not the own • In vitro, a major influence of i-light has been observed on different genotypes • In controlled conditions, the alliin content has appeared to be linked to the genotype • Garlic didn ’t seemed really affected by a carence of sulphur in natural conditions and in greenhouse • An increasing effect of the sulphur fertilisation on the bulbs alliin content had to be confirmed COOPD'OR R&D

  3. G&H October 2003, Münich WP3 / cultivation, P4 • To be confirmed in 2003 : • Influence of the intensity of the light • Effect of high dosis of sulphur (9 meq) • Effect of nitrogen combined to sulphur • Influence of S atmospheric COOPD'OR R&D

  4. G&H October 2003, Münich WP3 / cultivation, P4 Year 4 : what was done • 2 experiments In vitro : • Exp.III : Effect of various light conditions (intensity + quality) combined to 3 levels of sulphur in the medium CSO analysis achieved • Exp.IV : Effect of S nutrition combined to N nutritionIn vitro • 2 experiments in greenhouse : • 1.Effect of 3 nitrogen dosis (N, 2N and 3N) combined to a very high dosis of sulphur • 2. Observation and analysis on garlic providing from cloves grown in different sulphur nutrition levels in 2002CSO analysis in progress COOPD'OR R&D

  5. G&H October 2003, Münich WP3 / cultivation, P4 Experiments 2003  Observations - stage of the plant (in vitro : 1= growing / 2=beginning of bulbification / 3=maturation / 4=mature bulb) - number of green leaves - lenght of the highest green leaf  Measurements at harvest -weight, size and observation of the bulbs - alliin content (P5) GROWING BULB / ALLIIN COOPD'OR R&D

  6. 3 varieties : PRI + MES +MORl Gradient in SO4 0 3 9 (in macro-elements) meq 3 mediums (S- TS S++) 22-24°C - P = 16 hrs - F 1 month Cold induction 3°C - P = 10 hrs - F 2 months 22-24°C – P=16 hrs F = 9,50 µmol/s/m² 22-24°C – P=16 hrs F+i = 9,5 µmol/s/m² 22-24°C – P=16 hrs F = 20 µmol/s/m² G&H October 2003, Münich WP3 / cultivation, P4 In vitro experiment III-2003 • Effect of various light conditions (intensity + quality) and S nutrition

  7. G&H October 2003, Münich WP3 / cultivation, P4 In vitro experiment III-2003 - Growth observed in all the S treatments but clear influence of absence of S in macro-nutrients on the development of the vitro-plants - Very low percentage of bulbs well-formed for Printanor, except under F+i light conditions and presence of sulphur

  8. nd nd G&H October 2003, Münich WP3 / cultivation, P4 In vitro experiment III-2003 - Bulbs in treatment S- were quite big but soft • Alliin content Pri > Mor > Mes • AlCSO couldn ’t be detected in bulbs under S- treatment (Pri) • Effect of the S level (Pri) • No additional effect of i-light in S++ conditions

  9. G&H October 2003, Münich WP3 / cultivation, P4 Conclusion of experiment III • Positive effect of i-light confirmed for Morasol and Printanor in normal S conditions • No positive influence of the intensity of light and of the temperature in these experimental conditions • Influence of the sulphur dosis confirmed for Printanor • Vitro-plants appeared more sensitive to an absence of S in macro-nutrients than plants grown in natural conditions or in greenhouse

  10. varieties : PRI + MES + MORl + MORd Gradient in SO4 and nitrogen SO4 0 3 9 meq (in macro-elements) Nitrogen N 3N N 2N 3N N/2 N 2N 3N 9 medias 9 22-24°C - P = 16 hrs - F 1 month Cold induction 2 months 3°C - P = 10 hrs - F 22-24°C – P=16 hrs F+i – 9,50 µmol/s/m² G&H October 2003, Münich WP3 / cultivation, P4 In vitro experiment IV -2003 • Effect of S nutrition combined to N nutrition

  11. G&H October 2003, Münich WP3 / cultivation, P4 Greenhouse experiments 2003 (I) Effect of N combined to an excess of S • 3 varieties : Printanor, Morasol, Messidrôme • 3 mineral nutrition treatments : 3 levels of nitrogen combined to 9 meq of sulphur • N, 2N, 3N • 24 plants per treatment for each variety COOPD'OR R&D

  12. G&H October 2003, Münich WP3 / cultivation, P4 Greenhouse experiment I

  13. G&H October 2003, Münich WP3 / cultivation, P4 Greenhouse experiment I : 1st results on Messidrome => Less alliin measured when the dosis of N increases in presence of high dosis of S, but more GLUAlCS and IsoGLUAlCS COOPD'OR R&D

  14. G&H October 2003, Münich WP3 / cultivation, P4 Greenhouse experiment 2003 (II) Cloves from experiment 2002 COOPD'OR R&D

  15. G&H October 2003, Münich WP3 / cultivation, P4 Greenhouse experiment II : growth of Morasol (36plants per treatment)

  16. G&H October 2003, Münich WP3 / cultivation, P4 Year 4 : still to be done • Interaction SxN : • CSO analysis on experiment IV in vitro • CSO analysis on experiment I in greenhouse (Morasol, Printanor) • Influence of the S content of the bulb : • CSO analysis on experiment II in greenhouse (Printanor) • => Should be available at the beginning of november COOPD'OR R&D

  17. G&H October 2003, Münich WP3 / cultivation, P4 Milestones Year 4 · Experiments in vitro III and IV  Complementary experiment in greenhouse in year 4  Greenhouse cultivation of bulbs coming from the experiment 2002 COOPD'OR R&D

  18. G&H October 2003, Münich WP3 / cultivation, P4Deliverables P4 • DP 2 Chinese garlic powder to be analysed • DP 6 Garlic powder issued from various S-fertilisation in field condition • DP 14 Chemical distinction between garlic accessions when cultivated in various sulphur nutrition regimes and under distinct temperatures in vitro • DP 15 Physiological (growth traits) and chemical distinction (CSO) between accessions when cultivated in various sulphur nutrition regimes over two years • DP 19 Chemical distinction (CSO) between accessions when cultivated in various sulphur (greenhouse) and mineral nutrition regimes (in vitro) • DP 22  Paper on the interaction genotype x environment on the sulphur content of garlic plants grown in vitro , in greenhouse and in field conditions • DP 30 Paper on the effect of combined sulphur, selenium and nitrogen nutrition on garlic growth, flavour precursor content and on biological potential of garlic for disease prevention • DP 34  Chemical distinction (CSO) between garlic accessions when cultivated in various sulphur regimes (greenhouse) and mineral nutrition regimes (in vitro)

More Related