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Does my algae freeze?

Does my algae freeze?. Michele Stanley and John Day Scottish Association for Marine Science. Biodiversity Biological Resources. www.ccap.ac.uk. Value Pyramid for Algal Derived Products . (modified from Subitec Value Pyramid for Algae Product Markets in Bruton et al., 2009) .

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Does my algae freeze?

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  1. Does my algae freeze? Michele Stanley and John Day Scottish Association for Marine Science

  2. Biodiversity Biological Resources www.ccap.ac.uk

  3. Value Pyramid for Algal Derived Products • (modified from Subitec Value Pyramid for Algae Product Markets in Bruton et al., 2009)

  4. Case Study Microalgae-derived nutraceuticals Schlarb-Ridley & Parker (2013). A UK Roadmap for Algal Technologies for NERC/TSB Algal Bioenergy Special Interest Group.

  5. Traditional maintenance of algal cultures

  6. 5 µm Genotypic stability of algae Options available to assess genotypic stability • Phenotypic • Whole genome • Targeted gene • Culture stability • Morphological changes • Changes in productivity • Options available to conserve stability • Cryopreservation • Serial transfer

  7. Observed phenotypic changes • Loss of spine production • Apparently irreversible cell shrinkage in diatoms • Loss of ketocarotenoid production • Changes in apical cell structure in filamentous cyanobacteria • Loss of gas vacuole production • Change in phyco-biliprotein composition • Loss of alkaloid neurotoxin production

  8. Strain stabilityPhenotypePhaeocystisantarctica Gäbler-Schwarz et al. Cryoletters (in press)

  9. Why Long-term Preservation • Prevent phenotypic change/ loss of important attributes • Prevent genotypic change in conserved material • Prevent loss of strain • Reduce maintenance costs (staff & consumables

  10. Cryopreservation Protocol / methodological development Protocol validation Cryoinjury studies

  11. Evidence of genotypic & functional stability of a transgenic diatom Hipkin et al. (in press) J appl Phycol

  12. AFLP analysis of reference strain and cryopreserved E. gracilis- encapsulation, osmotic dehydration, 4 h desiccation, methanol treatment, control rate cooling and plunging into liquid nitrogen. Genotypic stability of cryopreserved Euglena gracilisCCAP 1224/5Z Harding et al. (2010) CryoLetters 31, 460-472.

  13. Post-cryopreservation functional/ phenotypic stability Hédoin et al. (2006) J. appl. Phycol. 18, 1-7.

  14. Post-cryofunctional/ phenotypic stability

  15. Conclusions • Rapid expansion algal cultures • Production GMOs on increase • Large sums of money being invested • Move from biofuels to biotechnology • Still needs to be underpinned • Recent EnAlgae survey • Demonstrated within some groups how little though is being given to this

  16. Scientific collaborators Glyn Stacey (NIBSC, UK) Thomas Mock & Rachel Hipkin (UEA, UK) Peter Kroth & Matthias Buhmann (Konstanz, D) Thomas Friedl & Maike Lorenz (SAG, D) Steffi Gäbler-Schwarz (AWI, D) Keith Harding & Erica Benson (DAMAR, UK) Josef Elster & JaromirLukavský (IB, CZ) AlenaLukešová (ISB, CZ) Katia Comte & RosiRippka (previously IP, F) Financial support & infrastructure KBBE- SeaBioTech

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