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100. anti-hnRNP B1 (U/mL). 10. 1. 1. 10. 100. 1000. anti-hnRNP A2 (U/mL).

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100

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  1. 100 anti-hnRNP B1 (U/mL) 10 1 1 10 100 1000 anti-hnRNP A2 (U/mL) SupplementaryFigure 1: Comparisonof anti-hnRNP A2 and anti-hnRNPantibodyanalyis: Testing 299 seraofpatientssufferingfrom rheumatoid arthritis, anti-hnRNP B1 antibodyanalysisbythenovel ELISA was correlatedwith anti-hnRNP A2 (RA33) antibodydetectionby a commerciallyavailable ELISA (HUMAN, Wiesbaden, Germany) (Spearman’srho = 0.209, 95% confidentialinterval: 0.098 – 0.315, p < 0.001).

  2. 55 kDa – 43 kDa – 30 kDa – Supplementary Figure 2: Immunoblot analysis of anti-hnRNP B1 antibody reactivity: Five sera of patients suffering from rheumatoid arthritis (1 – 5) and 5 sera of patients with systemic sclerosis (5 – 10) were tested for IgG reactivity to recombinant hnRNP B1 (in.vent DIAGNOSTICA GmbH, Hennigsdorf, Germany) blotted onto a nitrocellulose membrane after SDS polyacrylamide gel eletrophoresis. C – control serum (blood donor) P – Ponceau staining of the blotted hnRNP B1 polypeptide M 1 2 3 4 5 6 7 8 9 10 P C

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