Tissues

1. Tissues -four primary tissue types: 1. Epithelial 2. Connective 3. Muscle 4. Neural

2. Membranes -many body structures and surfaces are covered with membranes -superficial sheet of epithelial cells + underlying connective layer -cover & protect -four types: 1. serous 2. mucous 3. cutaneous 4. synovial

3. Serous membranes -a layer of connective tissue (areolar connective tissue) with a thin, single layer of epithelium on top of it -found lining internal cavities – e.g. heart chambers -found forming body cavities – e.g. peritoneum -also found covering internal organs within those cavities -serous = “watery” -serous membranes produce a thin, slippery, lubricating fluid serous membrane areolar connective tissue

4. Loose connective tissues: Loose Areolar tissue -cells are mainly fibroblasts, spaced far apart -matrix: mostly ground substance + collagen fibers, elastic fibers -strong and flexible but can be distorted due to its loose organization e.g. found beneath the dermis connecting it to muscle and bone

5. Simple Squamous Epithelium -tile-like cells - cells are thin, flat and irregular in shape = squamous -simple squamous epithelium - most delicate tissue in the body -found in protected regions where absorption occurs -many types: e.g. mesothelium - lines the abdomen e.g. endothelium - lines heart chambers and vessels

6. Mucous Membranes 2. Mucous membranes -line cavities that directly communicate with the exterior environment e.g. respiratory, urinary, reproductive, digestive -this membrane is also called a mucosa mucous membrane epithelium areolar connective tissue -covers, protects and moistens/lubricates -also absorbs, filters and secretes -exposed epithelial surface is covered with a layer of mucous made by single cells called goblet cells or large multicellular mucous glands

7. Mucous Membranes -epithelial layer can take many forms a. simple cuboidal or simple columnar epithelium in areas with no physical stresses b. pseudostratified columnar epithelium – looks layered but is a single layer of cells c. stratified squamous epithelial tissue in areas of physical stress d. transitional epithelium – stratified epithelium found in areas of stretching -connective tissue layer is areolar tissue = lamina propria --supports embedded blood vessels and nerves

8. Simple Cuboidal Epithelium -cells are “cubes” with large, round nuclei -found in regions of secretion and absorption e.g. kidney tubules pancreas & salivary glands - buffers & enzymes thyroid follicles - thyroid hormones

9. Simple Cuboidal Epithelium

10. Simple Columnar Epithelium -height is greater than their width -oval shaped nuclei at basal end of cell -found in areas of absorption and secretion -located in the gallbladder, larger ducts of exocrine glands, gastric pits of stomach -frequently the apical face is modified with microvilli e.g. intestinal lining = brush border -short-lived cells – replaced every 4 to 5 days -frequently found with goblet cells (intestine and stomach)

11. Simple Columnar Epithelium microvilli

12. Pseudostratified Columnar Epithelium - only a single layer despite looking like many layers of cells -nuclei are at varying levels - appearance of multiple layers -BUT basal face of every cell contacts the basement membrane -exposed apical surface typically bears cilia e.g. respiratory epithelium

13. Pseudostratified Columnar Epithelium • note the presence of cilia

14. basal lamina = impermeable layer of sugars and proteins; links epithelium to connective tissue in all membranes

15. Transitional Epithelium -stratified squamous epithelium that permits stretching -actual number of layers can be seen upon stretching -located in walls of the bladder, renal pelvis and the ureters -cells in deep layers can be columnar -transition to cuboidal towards surface

16. Transitional Epithelium

17. The Integumentary System -two major components: 1. cutaneous membrane = skin (epidermis, dermis) 2. accessory structures = hair, nails, exocrine glands

18. The skin: The Cutaneous Membrane • skin covers the entire body surface -including the anterior surface of the eye! -covers ~ 22 square feet -about 16% of total body weight • skin turns in at the mouth, nasal cavity, anus, urethral and vaginal openings – meets the mucous membranes lining these cavities • no “break” between the outer cutaneous and inner mucous membranes • 1st line of defense against pathogens

19. The Skin: functions 1. physical protection: protection from microbes, abrasion, heat 2. chemical protection – keratin - dryness of the epidermis; salt of sweat 3. regulation of water exchange: by sweating 4. regulation of body temperature: thermoregulation -by sweating & adjusting blood flow through the dermis 5. excretion of wastes -by sweating 6. nutrition – synthesis of vitamin D precursor -activated in skin, converted to calcitrol in liver 7. sensation: touch, pressure, vibration, pain & thermal 8. immune defense: Langerhans cells of the epidermis

20. Cutaneous membrane: the skin -covers the surface of the body -epithelial layer (keratinized stratified squamous epithelium) = Epidermis -underlying areolar connective tissue reinforced with dense irregular connective tissue = Dermis -underlying layer of areolar connective tissue and adipose tissue = Hypodermis

21. The skin: The Cutaneous Membrane • comprised of all four tissues: 1. epithelium – lines the inner and outer surfaces of body 2. connective – provides strength & resiliency 3. muscle – smooth muscle controls blood vessel diameter and controls movement of hairs 4. nervous – provides sensation and controls SM

22. Epidermis -stratified squamous epithelium - 5 layers maximum -four types of cells: 1. keratinocytes – make up the majority of the epidermis -epithelial cells that synthesize the protein keratin 2. melanocytes – cells for the synthesis of the light absorbing pigment melanin 3. Merkel cells – neurons that detect pressure 4. Langerhans cells – immune responses

23. Epidermis: layers - stratum germinativum or stratum basale: -inner most/deepest layer of the epidermis - contains basal cells called stem cells that differentiate into the keratinocytes and melanocytes of the epidermis -contains melanocytes for the production of melanin -neurons called Merkel cellsare found in hairless regions for detection of pressure and touch

24. Epidermis: layers - stratum spinosum: -keratinocytes of the stratum basale migrate up into this layer after dividing -made up of several layers thick of living keratinocytes -cells can divide - division of cells within this layer increases thickness of this layer -the more superficial cells in this layer are beginning the production of keratin -Langerhans cellsarefound in the deeper layers of this section - initiate immune responses to pathogens and to cancer

25. Epidermis: layers -stratum granulosum: -made up of keratinocytes migrating up from the stratum spinosum -cells contain large quantities of proteins (including keratin) – cytoplasm appears granular -the granules = keratohyalin granules - the cells die and dehydrate as they move up through this layer

26. Epidermis: layers -stratum lucidum: -covers the Str. Gran. -dead, flattened, densely packed cells filled with keratin -have a glassy appearance because they do not stain well -present only in the skin of fingertips, palms & soles of feet = THICK SKIN A. stratum corneum C. stratum spinosum B. stratum lucidum D. stratum granulosum C. stratum spinosum D. stratum granulosum E. stratum basale ONLY FOUND IN THE PALMS OF THE HAND SOLES OF THE FEET

27. Epidermis: layers - stratum corneum: -cornu = horn -makes up outer surface of both thick and thin skin -15-30 layers of flattened, dead, interlocking cells -large amounts of keratin are present – the tissue is said to be “cornified” -covered in secretions from oil glands to help moisturize the outer layer -but keratin makes this layer water-resistant - very dry to prevent growth of microorganisms

28. Epidermis: layers - stratum corneum: -most of this layer is hydrophobic -penetration is promoted by attachment to a lipid or dissolution in a lipid-based solution -transdermal drug patches – drugs are in oils or lipid-soluble carriers - moisturizing lotions – only penetrate few first layers of the corneum -takes 15-30 days to move from germinativum to corneum -cells will remain in corneum for an additional 2 weeks before being shed

29. Dermis -two major components: 1. papillary layer 2. reticular layer

30. Dermis Papillary Layer -about 1/5th thickness of dermis -loose areolar connective tissue + elastic fibers -numerous capillaries & sensory nerve endings -dermal projections into the epidermis =dermal papillae (papilla – “nipple-shaped mound) -some papillae contain Meissner’s corpuscles for touch -also free nerve endings – project into the epidermis - sensations of pain, warmth, itching dermal papilla

31. Dermis Reticular Layer (“little net”) -dense irregular connective tissue – interwoven collagen bundles plus elastic fibers in an irregular pattern -reticular layer contains blood vessels, nerves, hair follicles, sweat glands and sebaceous/oil glands -also contains lamellated corpuscles (Pacinian corpuscles) that detect deep touch and pressure Dense irregular connective tissue Dense regular connective tissue

32. PACINIAN CORPUSCLE MERKEL DISC

33. Fingerprints: epidermal ridges -formed from the stratum germinativum -extends down into the dermis -formed by the connections between dermal papillae and the epithelium -the contours of the skin follow these ridge patterns = fingerprints -function to increase the SA of the skin and increase friction

34. Skin colour • dermal blood supply: - hemoglobin bound to O2 – bright red in color so it gives pinkish cast to skin -when hemoglobin lacks O2 – bluish colour -this bluish skin colour = “cyanosis” -the thin skin of the lips and transparency of the nail enables us to see the blood in the peripheral circulation = red lips and pink nails -dermal blood supply comes from the larger blood vessels found in the subcutaneous layer called the hypodermis

35. Skin colour 2. pigmentation – two pigments: carotene and melanin -carotene= orange, yellow colour -derived from vitamin A  beta carotene - required for epithelial maintenance -carotene accumulates in keratinocytes -yellow color is very evident in the stratum corneum

36. Skin colour 2. pigmentation – two pigments: carotene and melanin -melanin= dark brown, black colour -synthesized from the amino acid tyrosine -melanin absorbs UV light and prevents damage to the keratinocytes of skin -produced and secreted by the melanocytes of the epithelium -melanin is transferred into the keratinocytes -light skin – melanin transfer occurs to keratinocytes only in the Str. germ. and Str. spinosum-dark skin - larger melanosomes and melanin transferred into the Str. granulosum dark skin = more active melanocytes – NOT more in number!

37. Hypodermis -also referred to as the subcutaneous layer or superficial fascia -connects the skin to underlying muscles or other organs -made up of: 1. loose areolar connective tissue 2. adipose tissue - “baby fat” - also contains elastic fibers for flexibility -contains large arteries and veins – supply the dermal plexuses with blood -the superficial layers of the hypodermis are the sites for drug injections - hypodermic

38. Loose Areolar tissue -cells: mainly fibroblasts, spaced far apart -some immune cells and adipocytes are also found -matrix: mostly ground substance + collagen fibers, elastic fibers -strong and flexible but can be distorted due to its loose organization e.g. found beneath the dermis connecting it to muscle and bone

39. Loose connective tissue: Adipose tissue -cells = adipocytes -cushions joints and organs -stores energy -insulates -in the hypodermis – adipose tissue is found intermixed with areolar tissue

40. Loose connective tissue: Adipose tissue -fat distribution changes with age and gender: -males – neck, upper arms, abdomen and lower back -females – breasts, abdomen, buttocks, hips and thighs

41. Wrinkles: reduction in the thickness of the dermis -loss of collagen in the dermal reticular layer -loss in dermal flexibility = wrinkles and sagging SOME FUN STUFF TO KNOW ABOUT SKIN Scars: from greek word schara (place of fire) -damaged dermis is replaced with tissue of inferior quality and rich in collagen -scars do not have sweat or oil glands and do not have hair -redness of the scar is due to inflammation and is not permanent - two common types: 1. hypertropic (red and raised, do not grow beyond boundaries of original wound 2. keloid – permanently growing scars – can lead to benign tumors - more common in darker skin, common on chest and shoulders Stretch marks: extensive and quick distortion of the dermis damages it -no recoil of skin after stretching -leads to breaking of elastic and collagen fibers in the dermis - replaced with new, poorly organized collagen Retin-A (tretinoin) : increases blood flow to the dermis -promotes dermal repair -decreases wrinkles and stretch marks

42. Accessory Structures of the Integumentary System Hair follicles Sweat glands Sebaceous/Oil glands Nails

43. Hair & Hair follicles • over all epidermal surfaces except soles of feet, palms of hand, sides of fingers and toes and portions of external genitalia • approx. 5 million hairs on the body • formed in and protected by structures known as hair follicles

44. Hair • Comprised of three major regions: 1. Hair bulb – contains the hair matrix – living cells undergoing division and keratin formation 2. Hair root – site of connection with arrector pili (smooth muscle) and a sebaceous gland (oil gland) 3. Hair shaft – portion of the hair above the sebaceous gland -portion of it is exposed above the skin • made up of an outer cortex and inner medulla of keratin -cortex – hard keratin - stiffness -medulla – soft keratin – flexible matrix

45. Hair • made up of an outer cortex and inner medulla of keratin -cortex – hard keratin - stiffness -medulla – soft keratin – flexible • outer most layer is made up of several layers of dead cells - cuticle

46. Hair follicle -found in the dermis -organ that makes the hair -surrounds and protects the hair bulb, root and shaft -made up of several layers

47. Exocrine Glands • skin is kept moist through the secretion of oil • skin is cooled through the evaporation of sweat • secretion is done via exocrine glands • exocrine gland – gland that secretes out onto an epithelial tissue such as the skin • most exocrine glands secrete via tubes called ducts

48. Exocrine Glands • exocrine secretions: 1. perspiration 2. digestive enzymes 3. milk 4. mucous 5. oil

49. Exocrine Glands • you can classify exocrine glands many ways • one way – by the consistency of what they secrete • e.g. serous, mucous, mixed • another way – by their structure • e.g. multicellular, unicellular • last way – by the mode of secretion • e.g. holocrine, merocrine, apocrine

50. exocrine gland types – consistency of secretion: 1. serous- watery fluid that contains enzymes e.g. saliva – parotid salivary gland 2. mucous - glycoproteins called mucins that absorb water to form a slippery mucus e.g. sublingual salivary gland 3. mixed- more than one type of gland cell -produces different types of secretions - mucus and serous e.g. submandibular salivary gland