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Figure S1.

Cotton plug. Filled with NaOH pellets. Syringe tube. 0.45 μ m syringe filter. BG-11 medium & cells. Figure S1. 12 C (1.1% 13 C). 49.6% 13 C. 98% 13 C. 10.8% 13 C. 250. 150. 100. 75. Band selected for in-gel digestion. 50. MW (kDa). 37. 25. 20. 15. 10. Figure S2.

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Figure S1.

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  1. Cotton plug Filled with NaOH pellets Syringe tube 0.45 μm syringe filter BG-11 medium & cells Figure S1.

  2. 12C (1.1% 13C) 49.6% 13C 98% 13C 10.8% 13C 250 150 100 75 Band selected for in-gel digestion 50 MW (kDa) 37 25 20 15 10 Figure S2.

  3. Figure S3.

  4. Synechocystis sp. PCC 6803 Synechococcus elongatus PCC 7942 12C (with natural 13C abundance 1.1%) 12C (with natural 13C abundance 1.1%) ν 1 ν 1 49.6% 13C 25.3% 13C 10.8% 13C 98% 13C 98% 13C 73.8% 13C 49.6% 13C ν 2 ν 2 Intensity ν 3 ν 3 Raman shift (cm-1) Figure S4.

  5. PCA result of RR spectra of Synechocystis sp. PCC 6803 single cells A 12C (with natural 13C abundance 1.1%) 10.8% 13C 25.3% 13C 49.6% 13C 73.8% 13C 98% 13C

  6. PCA result of RR spectra of Synechococcus elongatus PCC 7942 single cells B 12C (with natural 13C abundance 1.1%) 49.6% 13C 98% 13C Figure S5.

  7. Theoretical isotopic cluster distributions of the peptide 96% 13C 13% 13C 1.1% 13C 54% 13C 768.9 769.9 765.4 769.4 782.5 795.5 781.5 768.4 770.4 783.5 796.0 765.9 795.0 770.9 767.9 780.5 784.5 771.4 794.5 766.4 766.9 767.4 796.5 766.4 779.5 771.9 785.5 772.4 Intensity 794.0 772.9 765.9 778.4 766.9 773.4 786.5 793.5 797.0 777.4 767.4 793.0 797.5 792.5 m/z, Da m/z, Da m/z, Da m/z, Da Isotopic cluster distributions of the peptide 769.4 765.4 782.5 768.9 795.5 769.9 768.4 781.5 796.0 765.9 783.5 795.0 770.4 767.9 780.5 794.5 770.9 784.5 796.5 766.4 767.4 779.5 Intensity 771.4 794.0 785.5 766.9 771.9 793.5 778.4 766.9 793.0 772.4 766.4 792.5 797.0 786.5 765.4 792.0 765.9 777.4 772.9 767.4 791.5 797.5 773.4 m/z, Da m/z, Da m/z, Da m/z, Da 795 792 782 786 778 766 769 772 765.50 766.50 767.50 Figure S6

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