1 / 6

Term paper/ tubulin/ etc. : Lecture 6

6-1. Term paper/ tubulin/ etc. : Lecture 6. Term paper - getting started and finishing it: hints - tubulin folding pathway: complexity at its best. Term paper. You will have a better idea as to what you find interesting. Think of interesting questions that need to be addressed.

pravat
Download Presentation

Term paper/ tubulin/ etc. : Lecture 6

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 6-1 Term paper/ tubulin/ etc.: Lecture 6 Term paper - getting started and finishing it: hints - tubulin folding pathway: complexity at its best

  2. Term paper You will have a better idea as to what you find interesting Think of interesting questions that need to be addressed Search and read the latest literature on that topic Write 5-page Term paper according to guidelines Pick out a topic that is broad (biogenesis, degradation or related topic) then narrow down a subject by reading a review or two (I will suggest some reviews) Narrow down subject even further, e.g., to a protein or a process 6-2 Patiently wait until the end of next week

  3. Tubulin assembly 6-3 α tubulin β tubulin αβ-heterodimer assembled microtubule http://cellbio.utmb.edu/cellbio/microtubule_structure.htm

  4. FtsZ crystal structure 6-4 - archaeal FtsZ is the homologue of eukaryotic tubulin - first tubulin to be crystallized; shows the power of crystallizing proteins from different sources - structure of alpha-beta-tubulin heterodimer is now also known (TREMENDOUSLY difficult to obtain; used electron diffraction) - same group also published structure of MreB, a bacterial protein that forms filaments and has a structure that resembles actin van den Ent et al. (2001) Prokaryotic origin of the actin cytoskeleton. Nature413, 39-44. Lowe and Amos (1998) Crystal structure of the bacterial cell-division protein FtsZ. Nature391, 203-6.

  5. 6-5 Tubulin folding pathway Tian et al. (1997) J. Cell Biol.138, 821-32.

  6. Crystal structure of Rbl2p, the cofactor A for b-tubulin folding 6-6 - Rbl2p is the yeast homologue of cofactor A - binds monomeric b-tubulin - suppresses deleterious effects of excess b-tubulin in vivo - competes with a-tubulin in the b-tubulin heterodimer (therefore binds near native state?) - surface mostly hydrophilic; dimer interface has hydrophobic residues - may act as a dimer

More Related