230 likes | 332 Views
Determine new application spaces in the sequencing platform using visions systems. Jessica Perez Summer Research Program in Genomics Massachusetts Institute of Technology Summer 2007. Outline. Approach and Goals Vision Systems Potential Application Spaces Demonstrated Application Spaces.
E N D
Determine new application spaces in the sequencing platform using visions systems Jessica Perez Summer Research Program in Genomics Massachusetts Institute of Technology Summer 2007
Outline • Approach and Goals • Vision Systems • Potential Application Spaces • Demonstrated Application Spaces
Project Overview • Approach: use Cognex Smart Sensor and Intellect 1.4.0 software as a model to demonstrate the capabilities of vision systems • Goals: • Explore capabilities of software • Look for application spaces • Take application and prove principal
Vision Systems Software Capabilities Lighting Post Processing
Software Capabilities Blob Identification 1D Bar Scanning Flexible Flaw Detection
User Interface Tool Box Viewing Screen Result Table
Blob Identification Lighter Objects FAILED Darker Objects Meet Parameters
1D Code Reader Numerical Code Read PASS
Flaw Detection Model
Additional Tests Counting Filtering TOOLS Measurement Optical Character Recognition Reader 2D String Reader
Lighting Geometry Filtering Intensity Answer.com
Common Lighting Techniques Bright Field Dark Field Back Lighting From Vision Light Tech
Post Processing MatLab Histogram Filter Before After
Potential Application Spaces Process: Solexa Screening Consumer: Glass Tubes
Process: Solexa Screening • Quality Control Check in the Solexa Sequencing Process Flowcells Clusters
Proof of Principle • Blobs represent clusters of DNA
Consumer : Test Tube Probe Sonication Sonic Waves DNA
Backlighting BEFORE Detection of Dimensions and Shape of Transparent Objects AFTER Images by Keyence-Engineering Knowledge
Setup Components LED Strobe Light Diffuser Magnification Lens
Camera Strobe Light Tubes Mask Setup
Finish Product Tube under Backlighting Distance to be measured
Conclusion • Vision systems have unlimited potential • Implementation of vision systems will improve the efficiency at various stages of the sequencing process.
Technology Development Group Eva Markiewicz Shailendra Yadav Filip Rege Andrew Barry Todd Sparrow Tracey Honan Pat ___ Directors of Education Shawna Young Dedric Carter Eric Lander Broad Institute Acknowledgements