1 / 26

CD56 Expression in Bone and Osteoblastic Neoplasia: A Novel Osteoblast Marker?

CD56 Expression in Bone and Osteoblastic Neoplasia: A Novel Osteoblast Marker?. D.E. Hughes, R. Deb, D.C.Mangham, V.P. Sumathi and R.J. Grimer Royal Orthopaedic Hospital, Birmingham, U.K. CD56/NCAM. Cell adhesion molecule of the immunoglobulin superfamily

rafe
Download Presentation

CD56 Expression in Bone and Osteoblastic Neoplasia: A Novel Osteoblast Marker?

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. CD56 Expression in Bone and Osteoblastic Neoplasia:A Novel Osteoblast Marker? D.E. Hughes, R. Deb, D.C.Mangham, V.P. Sumathi and R.J. Grimer Royal Orthopaedic Hospital, Birmingham, U.K.

  2. CD56/NCAM • Cell adhesion molecule of the immunoglobulin superfamily • Involved in homotypic cell-cell interactions • Possible role in neural morphogenesis • Restricted tissue distribution

  3. CD56 expression in normal tissues • Neurons, gangliocytes • Plasma cells • Natural killer cells

  4. CD56 expression in tumours • Myeloma • Neuroendocrine carcinomas • Neuroblastoma • Phaeochromocytoma • Some glial tumours • NK-type LGL

  5. Previous evidence of CD56 expression in osteoblast lineage • Expressed by calvarial cells in vitro • Described in association with CD56 expression in myeloma in osteoblasts • Reported in osteosarcomas • Not expressed in Ewing’s sarcoma • Not expressed in chondrosarcoma

  6. Aim of study • To assess CD56 expression in normal cells of the osteoblast lineage • To assess CD56 expression in tumour-like lesions in which bone is formed • To assess CD56 expression in osteoblastic neoplasms

  7. Methods • Cases selected from Royal Orthopaedic Hospital paraffin archive to represent a broad range of normal bone, reactive conditions, tumour-like conditions and osteoblastic tumours • CD56 expression assessed by a standard immunohistochemical approach

  8. Appendix CD56 staining of neural cells Immunohistochemistry • Novacastra anti- CD56 clone 1B6 at 1:50 • High temperature antigen retrieval in citrate/EDTA • Detection by Enivision system

  9. Case selection – benign • Normal/reactive bone (31 cases) • Quiescent bone • Callus, periosteal reaction • Benign bone tumours and tumour-like conditions (24 cases) • Aneurysmal bone cyst • Fibrous dysplasia • Osteofibrous dysplasia • Non-ossifying fibroma • Desmoplastic fibroma • Osteoid osteoma • Osteoblastoma • Giant cell tumour

  10. Case selection – malignant • Malignant osteogenic tumours (23 cases) • High grade intramedullary osteosarcoma • Telangiectatic osteosarcoma • Intermediate grade chondroblastic osteosarcoma • Parosteal osteosarcoma • Low grade central osteosarcoma • Undifferentiated recurrences of known osteosarcomas • Non bone-forming intra-osseous sarcomas

  11. Results: normal bone (1) Strong expression in lining cells and osteoblasts

  12. Weak expression in stromal cells adjacent to osteoblast seams Osteocytes Predominantly negative Results: normal bone (2)

  13. No expression in cartilage No expression in fibroblasts Strong expression in osteoblasts Results: callus

  14. No expression in outer fibrous layer Strong expression in bone-forming areas Results: periosteal reaction

  15. Strong expression on Surface layer of septal structures Background stromal Population negative Results: aneurysmal bone cyst

  16. Results: fibrous dysplasia Spindle cell component positive – most strongly adjacent to areas of bone formation Similar results in osteofibrous dysplasia

  17. Results: non-ossifying fibroma Some expression in spindle cells adjacent to bone Similar results in desmoplastic fibroma

  18. Results: osteoid osteoma Nidus osteoblasts positive Similar results in osteoblastoma

  19. Focal weak expression by spindle cell component Osteoclasts negative Results: giant cell tumour

  20. Most tumour cells positive Strong expression in osteoblastic areas Loss of expression in chondroblastic areas Results: high grade osteosarcoma

  21. Results: parosteal osteosarcoma Variable expression in spindle cell component – stronger adjacent to areas of osteoid Similar results in low grade central osteosarcoma

  22. Focal expression In undifferentiated Tumour cells Results: telangiectatic osteosarcoma

  23. Areas of expression in the absence of osteoid formation Results: undifferentiated recurrence of high grade osteosarcoma Similar results in some undifferentiated intra-osseous sarcomas

  24. Results - summary by cell type: non-neoplastic • Normal osteoblasts – always positive • Normal osteocytes – mostly negative • Normal periosteal spindle cells – positive in areas of active bone formation • Normal bone marrow stromal cells - positive in areas of active bone formation

  25. Results - summary by cell type: neoplastic • Neoplastic osteoblasts – consistently positive • Neoplastic osteocytes – variably positive • Neoplastic spindle cells with osteogenic potential – variably positive • Neoplastic spindle cells without evidence of osteogenic potential – sometimes positive

  26. Conclusions • CD56 is consistently strongly expressed by normal and neoplastic osteoblasts • CD56 expression also occurs in non-osteogenic spindle cells in bone and bone lesions – it may not be a specific marker of osteoblastic potential for diagnostic purposes • CD56 may be a biologically important molecule in osteoblast function

More Related