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Oligonucleotides used in this work .

Supplementary materials. Oligonucleotides used in this work . Figure. S1. a Amino acid sequence of coding regions of the Streptavidin-PGL35- HCV core protein. Figure. S1. b Amino acid sequence of coding regions of the Streptavidin-PGL35- HIVp24.

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Oligonucleotides used in this work .

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  1. Supplementary materials Oligonucleotides used in this work.

  2. Figure. S1. a Amino acid sequence of coding regions of the Streptavidin-PGL35-HCV core protein

  3. Figure. S1. b Amino acid sequence of coding regions of the Streptavidin-PGL35-HIVp24

  4. Figure. S2Alignment of amino acid sequences for PGL35 from Arabidopsis, tobacco, rice, tomato, Medicago and potato. The N-terminal transit peptide amino acids were removed and sequence alignment was performed by CLUSTALW.

  5. (a) 2.5 WT 1.1 (b) Figure. S3 Phenotype of wild- type and transplastomic lines (a) The wild-type and transplastomic seedlings pVSB1 (1.1), pVSB2 (2.5) 7 days after sowing on soil. (b) Wild-type, pVSB1 (1.1) and pVSB2 (2.5) transplastomic plants were grown on soil for 4 weeks. (c) 12-week-old plants (T1 generation) from transplastomic lines pVSB1 (1.1) and pVSB2 (2.5) grown in a greenhouse are phenotypically indistinguishable from wild-type tobacco WT 2.5 1.1 (c) 2.5 WT WT 1.1

  6. PGL25 PGL29 Thylakoid PG/thylakoid Isoelectric point (pI) PG core Strep-PGL35-HIVp24 PGL45 PGL34-YFP PGL34 PGL35 PGL40 Hydrophobicity (GRAVY Index) Figure. S4 Plastoglobulin and Streptavidin-PGL35-HIVP24 fusion protein localization is determined by isoelectric point and hydrophobicity. The graph adopted from (Lundquist et al. 2012).

  7. pVSB2 (2.5) WT TC TCTM S kDa 116 97 66 Amidoblack 44 31 Anti-PGL40 Anti-RLSU Anti-Lhcb2 Figure. S5 Total chloroplast (TC) were isolated from wild type (WT) and pVSB2 (2.5). The transplastomic pVSB2 (2.5) chloroplasts were lysed and separated into total membranes (TM) and stroma (S). 10µg of protein were resolved by SDS-PAGE, transferred to nitrocellulose, and immunoblottedwith antisera to the proteins as indicated. PGL40 (plastoglobule marker), RLSU (stroma marker) and Lhcb2 (thylakoid marker).

  8. TableS1Chlorophyll content and fluorescence in leaves of tobacco wild-type and Streptavidin-PGL35-fusion lines pVSB1 (1.1) and pVSB2 (2.5) Fv/Fm – maximum photochemical quantum yield of photosystem II

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