CYTOTOXICITY EFFECT OF RESVERATROL OLIGOMERS AND THEIR DERIVATIVES AGAINST HUMAN CANCER CELL LINES

1. CYTOTOXICITY EFFECT OF RESVERATROL OLIGOMERS AND THEIR DERIVATIVES AGAINST HUMAN CANCER CELL LINES Sri Atuna, Nurfina Aznama, Retno Arianingruma, Takaya Yb, Niwa Masatakeb a Department Chemistry education, Universitas Negeri Yogyakarta, Karangmalang, Depok, Sleman, Yogyakarta, 55281 b Faculty of Pharmacy, Meijo University, Tempaku, Nagoya, Japan METHODE OF RESEARCH Sample: milled dried stem bark Hopea is one the main genus of Dipterocarpaceae, consisting of approximately 100 species and widely distributed in Indonesia specially in Kalimantan The local name is “ cengal, merawan hitam or pengarawan”. The plant ussually can be used as material building, plywood etc. This family of plant is known to produce a variety of resveratrol oligomer These structures are very interesting and showed interesting biological activity, such as antibacterial, anticancer, antihepatotoxic, and anti-HIV Extraction by organic solvent : acetone or methanol EXTRACT By fractionated (VLC) Fr A Fr B Fr C Fr D Repeated Chromatography elusidasion structure by Spectroscophy methode (UV, FT-IR, NMR, MS) Pure compounds S-1, S-2, … etc Methylation & Acethylation BIOACTIVE COMPOUNDS STRUCTURE MOLECULE DERIVATIVES COMPOUNDS Isolated compounds from H. odorata (3.8 Kg) Isolated compounds from H. mengarawan (5 Kg) Isolated compounds from H. nigra (3.5 Kg) Vaticanol G (70 mg) Hopeaphenol (1500 mg) Heimiol A (200 mg) Balanocarpol (300 mg) Balanocarpol (300 mg) vaticanol G (200 mg) Ampelopsin H (250 mg) Hemlesyanol C (120 mg) vaticanol B (200 mg) Methode identification structure by modern spectroscophy Spectrum UV and IR of Vaticanol B Spectrum 1H-1H COSY NMR of vaticanol B Spectrum 1H and 13C NMR of vaticanol B Cytotoxicity test byRaji cell lines Cytotoxicity test byHela S3 cell lines Hela S3 cell lines before experiment Hela S3 cell lines after experiment Raji cell lines before experiment Raji cell lines after experiment Table 1. The cytotoxic effects of resveratrol oligomers and their derivatives in human cancer cell lines Conclusion In this paper we conclusion that isolated compound from steam bark of H. mengarawan, H.odorata and H. nigra have cytotoxic effect against Raji, HeLa-S3, and Myeloma cell lines. Vaticanol B (4) and ampelopsin H (6) give the highest cytotoxic effect against HeLa-S3 and Raji, while the derivative resveratrol, methyl and acethylbalanocarpol and hopheaphenol showed not active. Acknowledgements This work has been supported by competitive grant (Insentif Riset Dasar, Ristek- 2008), Ministry Reseach and Technology, Republic of Indonesia. The authors are grateful to the experimental Garden in Carita, Pandeglang, Banten, Indonesia and Herbarium Bogoriensis for supported the sample and identification of the plant specimen. *Note : not finished yet e-mail : Atun_1210@yahoo.com