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Helicobacter pylori SabA Adhesin in Persistent Infection and Chronic Inflammation

This scientific article discusses the role of the Helicobacter pylori SabA adhesin in persistent infection and chronic inflammation of the human stomach. The adhesin binds to fucosylated blood group antigens and is involved in cell attachment, tissue colonization, and invasion. The article presents experimental findings and images illustrating the binding of H. pylori to specific antigens and the induction of expression of certain antigens during infection. The implications of this research in understanding the development of gastric adenocarcinoma are also discussed.

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Helicobacter pylori SabA Adhesin in Persistent Infection and Chronic Inflammation

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  1. Helicobacterpylori SabA Adhesin in Persistent Infection and Chronic Inflammation Science 297, 573-578, 2002

  2. Helicobacter pylori is a Gram-negative bacterium that colonizes the human stomach, mainly persisting within the gastric mucus layer. • Chronic infection is associated with the development of gastric adenocarcinoma, and H. pylori was recently defined as a class I carcinogen.

  3. Adhesins are microorganisms’ surface protein that interact with glycoproteins, proteoglycans, and glycolipids.The functions of microoragnisms’ adhesins are cell attachment, tissue colonization, and invasion.The best defined H. pylori adhesin found to date is Leb blood group antigen binding adhesin (BabA).BabA bind to fucosylated blood group antigens Lewis B (Leb) and H-1.

  4. Representive of Lewis structure

  5. babA1A2 (+) BabA1A2 (-) Infected cells Leb mAb stain Inhibition with Leb sLex mAb stain Inhibition with sLex Noninfected cells sLex mAb stain babA1A2 (+) BabA1A2 (-) Fig. 1 ThesLex antigen confers adherence of H. pylori-infected (strain Wu 12) human gastric mucosa.

  6. H/E stain of transgenic Leb mouse gastric mucosa babA1A2 (+) BabA1A2 (-) sLex mAb stain Inhibition with sLex Fig. 2A

  7. Chemically stained Probed with sLex mAb 1. Calf brain 2. Human neutrophile granulocytes 3. Desialylation of #2 4. Adenocarcinoma 5. Desialytion of #4 6. sdiLex GSL 7. sLea GSL 8. S(mono) Lex GSL 9. Leb GSL Probed with babA1A2(+) Probed with babA1A2(-) Fig. 2B

  8. Fig. 2C

  9. Fig. 3 H. pylori binds to sialyted antigens

  10. Leb mouse gastric mucosa Human gastric mucosa Fig. 3 H. pylori binds to sialyted antigens

  11. Fig. 4 Infection induces expression of sLex antigens

  12. Fig. 5 Retagging of SabA

  13. J99 sabA mutant Control Preatment with Leb Fig. 5B

  14. J99 sabA mutant J99 wild type J99 babA mutant J99 babA/sabA mutant Fig. 5C

  15. Fig. 6

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