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Thiol quantification. Professor : Chau , Lai-Kwan Student : Liu, Wan-Chun Date : 2013/11/08. Method. 2% MPDMS, or 2% MPTMS. plasma. 0 .004mM DTNB. Glass slide. UV. 12hr. 15min. 16hr. Optimization - concentration of DTNB. Mechanism of E llman’s reaction.
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Thiol quantification Professor:Chau, Lai-Kwan Student:Liu, Wan-Chun Date:2013/11/08
Method 2%MPDMS, or 2% MPTMS plasma 0.004mMDTNB Glassslide UV 12hr 15min 16hr
Verify by strong/weak reducing agents Strong reductant : DTT Weak reductant: 2-mercaptoethanol
Standard curve (r2=0.998)
Thiolcoverage on glass surface 5 cm (SH/nm2) 0.2 cm 0.8 cm
Thiol coverage on glass surface 0.7 nm 0.4nm
Future work • Gold coverage on MPDMS/MPTMS modified glass surface.