Poster Presentation

Poster Presentation Lisa Russell Anthony V Moorman Roy Russell Richy Hetherington ElizabetaMukaetova-Ladinska

Overview of Session • Talk • Review communication modes in science • Advantages and disadvantages of posters • Features of a good poster • Tips and hints • Interactive section • Review posters in groups; focussing on different elements of the posters around the room (15-20min) • Identify 3-4 key issues with respect to the element in question • Short group presentation (2-3 minutes per group) • Questions

Modes of Communication in Science • Written (i.e. paper / article) – good for detail; time to digest; possible to include complex material Clear beginning, middle and end; Reader can combine with other sources of information to clarify anything that is not clear. • Oral/Spoken/Verbal – good for explaining; can get feedback from listener(s) Limited attention span but possible to repeat or explain technical jargon appropriate to audience. • Visual/Poster – initial impact; memorable image Little or no personal contact; limited viewing time; competing for attention.

When are posters used? • Meetings/Conferences • General, Specialist, Clinical • Large, Small • National, International • Open days • Entertaining fundraisers • Attracting staff, students • Informing general public • Site Visits / Visits from Funding Bodies • Corridor decoration • Coursework

Pros and Cons of the SpokenPresentation PROS • Large audience • High visibility of speaker • Opportunity to stress specific points • Easier to prepare • Last-minute changes possible (not always an advantage) • Slides reusable CONS • Nerve-wracking • Not all audience interested • Often few questions/little discussion

Pros and Cons of the Poster Presentation PROS • Good for certain kinds of data • Attracts interested viewers • Opportunity for discussion (2 way) • Good if you are a nervous speaker • Good if English is not your first language • Displayed all day / whole conference – more exposure CONS • Time consuming to make (?) • Cost • May miss viewing parallel posters (?) • Not as glamorous as talks

Poster Sessions What is in it for the viewer? • Can be selective • Opportunity to meet presenter - ask detailed questions • ask simple questions • chance to mention your own interests • Can eavesdrop / join in discussions • Time to think about content • Can take notes, get methods, references

A good poster will rapidly allow the reader to answer the questions: • What is this about? Title, picture(s) • What are they doing? Aims • What is the bottom line ? Conclusion • How did they do it? Methods, Results • Who are these guys? Names, Presenter • How can I find out more? Contact details, references Grab attention | Give information | Memorable message

Visual impact Title Layout Readability Use bullet points Poster Style – things to consider

Title • Possibly the most important aspect of the poster. • First thing viewers will read (sometimes only!) • Short but needs to capture the essence of the poster • Choose your wording carefully and remember that the more words you have, the more space they will take up.

An Investigation Into The Contributory Factors That Improve The Visual Presentation Of Scientific Results In A Conference Scenario. An Investigation Into The Contributory Factors That Improve The Visual Presentation Of Scientific Results In A Conference Scenario. AN INVESTIGATION INTO THE CONTRIBUTORY FACTORS THAT IMPROVE THE VISUAL PRESENTATION OF SCIENTIFIC RESULTS IN A CONFERENCE SCENARIO. An Investigation Into The Contributory Factors That Improve The Visual Presentation of Scientific Results In a Conference Scenario An investigation into the contributory factors that improve the visual presentation of scientific results in a conference scenario

An investigation into the contributory factors that improve the visual presentation of scientific results in a conference scenario Remove redundant words Factors that improve visual presentation of scientific results (note how you can now make the text bigger)

Size matters Relationship between size of font used in a figure and the ease with which it can be read at a distance of 1 m assessed using a ten-point scale (readability )

Poster Layout • Landscape better than portrait 3 columns – reduces line length

First Impressions Count

Know your audience • Expert, semi-expert, patients, fund-raisers, general public • THINK about content, text and terminology • Avoid acronyms – even ones that seems obvious to you • No jargon / Do not assume knowledge • Always ask yourself - do they need to know this piece of information in order to understand my message?

Tables – keep them simple!

Tables – Focus on the key data.

Keep figures simple too Figure 3. Distribution of the percentage of positive nuclei in patients with recurrent IGH@ partner genes Figure 2. Percentage of positive nuclei scored in patients with recurrent IGH@ translocations. When comparing the percentages of positive nuclei observed in patients with recurrent IGH@ translocations (known or unknown partner genes), the involvement of certain partner genes was present in the majority of cells: BCL2, CEBPA, CEBPB and CRLF2. Together patients with involvement of these genes had a median translocation population of 79%. This was reduced in patients with ID4 involvement to 65% and further reduced to 30% in patients with IGH@-CEBPE. Patients with IGH@-CEBPD translocations tended to have a wind range of translocation positive nuclei with some as low as 21% and one at 100%. Boxes denote the range of positive nuclei scored, with extending thin black lines marking outliers. The thick black line within the boxes denotes the median percentage of positive nuclei scored per partner gene.

2020 IL-18 and Immune Responses to Periodontal PathogensLeah Jamieson (supervisors Neil Foster and John Taylor)Oral Microbiology and Host Responses Group, School of Dental Sciences, University of Newcastleemail: L.M.Jamieson@ncl.ac.uk Aims • To establish whether human THP-1 monocytes produce IL-18 when stimulated with Porphyromonas gingivalis LPS • To examine the relationship between pro-inflammatory • IL-18 and anti-inflammatory IL-18 BPa A novel ELISA was developed to detect IL-18 BPa. Anti-IL-18 BPa was added to the supernatants of P. gingivalisstimulated cells to investigate the effect on IL-18 concentration. The addition of anti-IL-18 BPa significantly (P <0.05) increased the concentration of IL-18 assayed by the ELISA (Figure 4). Results Introduction * * Both P. gingivalis and E. coli LPS induced IL-18 secretion in THP-1 monocytes as demonstrated by ELISA and Western blotting (Figure 1 & 2) as well as IL-18BPa (Figure 3). The latest UK Dental Health Survey revealed that 40-45% of adults have moderate destructive periodontal disease, with 5-10% of cases being severe. Periodontal disease is a result of excessive immune responses to dental plaque bacteria which destroy the supporting tissues of the teeth, leading to loss of dental function. Cytokines play a central role in inflammatory processes that influence the progression of periodontal disease and its clinical outcome. Interleukins (IL) are a group of cytokines produced primarily by leukocytes. IL-18 has pro-inflammatory properties. It induces production of interferon (IFN)-γ which has a major role in the activation of cells of the immune system. IL-18 binding protein (BPa) strictly controls IL-18 activity by blocking binding to the IL-18 receptor on target cells and IL-18 BPa expression is stimulated by IFN-γ. This is an important regulatory circuit as overproduction of IFN-γ causes tissue pathology. However, there is no information on IL-18 in the pathogenesis of this disease. Figure 4: Addition of anti-IL-18 BPa to P. gingivalis LPS stimulated supernatants (6h) significantly increases IL-18 detection by ELISA (N= 3) (* = P< 0.05) Discussion The results indicate that significant concentrations of IL-18 and IL-18BPa are produced by human THP-1 cells within 6 hours of initial exposure to P. gingivalis LPS. Antibodies to IL-18BPa increased the amount of free IL-18 in LPS-stimulated THP-1 cultures indicating that IL-18BPa normally binds IL-18 secreted from THP-1 monocytes. The balance between IL-18 and IL-18 BPa may be important in regulating IL-18 activity and may play a role in the host response to plaque bacteria, and possibly progression and severity of periodontal disease. This work also indicates that antibodies or binding proteins could be used to modify immune responses involving cytokines and therefore be used therapeutically in the treatment of periodontal disease. Clinical signs of destructive periodontal disease Figure 1: ELISA analysis to quantitate IL-18 secreted by THP-1 cells exposed to LPS from P. gingivalis and E. coli. Cultures with PMA and PBS served as positive and negative controls respectively (N= 6) (* = P <0.05) P. gingivalis E. coli - ve → Figure 2: Western blot of IL-18 protein in supernatants from THP-1 cells exposed to LPS from P. gingivalis and E. coli (6h) * * * Conclusions * * Methods • The balance between IL-18 and IL-18 BPa may be important in immune responses to periodontal bacteria • This system may represent a novel immunoregulatory circuit in periodontal disease and a potential therapuetic target Cells from the pro-monocyte cell line THP-1 were treated with Vitamin D3 (0.1 μM) to induce differentiation into monocytes. Differentiation was confirmed by the ability of the cells to adhere to the plastic plate. THP-1 monocytes were cultured with lipopolysaccharide (LPS) from the periodontal bacterium P. gingivalisand the enterobacterium E. coli for 6 and 24 hours. The concentration of protein in each sample was determined by a protein assay and IL-18 concentrations were measured using an ELISA assay kit and detected via a Western Blot. PBS Pg PMA Ec Acknowledgements Figure 3: ELISA analysis of IL-18 BPa secreted by THP-1 cells exposed to LPS from P. gingivalis or E. coli. Cultures with PMA and PBS served as positive and negative controls respectively (N =3) (*= P< 0.05) This project was supported by a Wellcome Trust Vacation Scholarship

2020 IL-18 and Immune Responses to Periodontal PathogensLeah Jamieson (supervisors Neil Foster and John Taylor)Oral Microbiology and Host Responses Group, School of Dental Sciences, University of Newcastleemail: L.M.Jamieson@ncl.ac.uk Aims • To establish whether human THP-1 monocytes produce IL-18 when stimulated with Porphyromonas gingivalis LPS • To examine the relationship between pro-inflammatory • IL-18 and anti-inflammatory IL-18 BPa A novel ELISA was developed to detect IL-18 BPa. Anti-IL-18 BPa was added to the supernatants of P. gingivalis stimulated cells to investigate the effect on IL-18 concentration. The addition of anti-IL-18 BPa significantly (P <0.05) increased the concentration of IL-18 assayed by the ELISA (Figure 4). * * Results Introduction Both P. gingivalis and E. coli LPS induced IL-18 secretion in THP-1 monocytes as demonstrated by ELISA and Western blotting (Figure 1 & 2) as well as IL-18BPa (Figure 3). The latest UK Dental Health Survey revealed that 40-45% of adults have moderate destructive periodontal disease, with 5-10% of cases being severe. Periodontal disease is a result of excessive immune responses to dental plaque bacteria which destroy the supporting tissues of the teeth, leading to loss of dental function. Cytokines play a central role in inflammatory processes that influence the progression of periodontal disease and its clinical outcome. Interleukins (IL) are a group of cytokines produced primarily by leukocytes. IL-18 has pro-inflammatory properties. It induces production of interferon (IFN)-γ which has a major role in the activation of cells of the immune system. IL-18 binding protein (BPa) strictly controls IL-18 activity by blocking binding to the IL-18 receptor on target cells and IL-18 BPa expression is stimulated by IFN-γ. This is an important regulatory circuit as overproduction of IFN-γ causes tissue pathology. However, there is no information on IL-18 in the pathogenesis of this disease. Figure 4: Addition of anti-IL-18 BPa to P. gingivalis LPS stimulated supernatants (6h) significantly increases IL-18 detection by ELISA (N= 3) (* = P< 0.05) Discussion The results indicate that significant concentrations of IL-18 and IL-18BPa are produced by human THP-1 cells within 6 hours of initial exposure to P. gingivalis LPS. Antibodies to IL-18BPa increased the amount of free IL-18 in LPS-stimulated THP-1 cultures indicating that IL-18BPa normally binds IL-18 secreted from THP-1 monocytes. The balance between IL-18 and IL-18 BPa may be important in regulating IL-18 activity and may play a role in the host response to plaque bacteria, and possibly progression and severity of periodontal disease. This work also indicates that antibodies or binding proteins could be used to modify immune responses involving cytokines and therefore be used therapeutically in the treatment of periodontal disease. Figure 1: ELISA analysis to quantitate IL-18 secreted by THP-1 cells exposed to LPS from P. gingivalis and E. coli. Cultures with PMA and PBS served as positive and negative controls respectively (N= 6) (* = P <0.05) Clinical signs of destructive periodontal disease P. gingivalis - ve E. coli → Figure 2: Western blot of IL-18 protein in supernatants from THP-1 cells exposed to LPS from P. gingivalis and E. coli (6h) * * * * * Conclusions Methods • The balance between IL-18 and IL-18 BPa may be important in immune responses to periodontal bacteria • This system may represent a novel immunoregulatory circuit in periodontal disease and a potential therapuetic target Cells from the pro-monocyte cell line THP-1 were treated with Vitamin D3 (0.1 μM) to induce differentiation into monocytes. Differentiation was confirmed by the ability of the cells to adhere to the plastic plate. THP-1 monocytes were cultured with lipopolysaccharide (LPS) from the periodontal bacterium P. gingivalis and the enterobacterium E. coli for 6 and 24 hours. The concentration of protein in each sample was determined by a protein assay and IL-18 concentrations were measured using an ELISA assay kit and detected via a Western Blot. Pg Ec PBS PMA Acknowledgements Figure 3: ELISA analysis of IL-18 BPa secreted by THP-1 cells exposed to LPS from P. gingivalis or E. coli. Cultures with PMA and PBS served as positive and negative controls respectively (N =3) (*= P< 0.05) This project was supported by a Wellcome Trust Vacation Scholarship

Corporate Visual IdentityNewcastle University Policy and Regulations Corporate Visual IdentityNewcastle University Policy and Regulations http://www.ncl.ac.uk/cvi-support/

Do NOT forgot your sponsor!

Getting noticed at meetings

Conference layout

Look at Me!!!!! 400 posters 90 mins 0.225 min/poster 13.5 sec/poster

Typical Instructions for Poster Presentations • One poster board will be provided for you. The poster board is approximately 4 feet high and 8 feet wide. • We will provide a printed “number,” identifying each poster board. Push pins will also be provided. • One or two authors should be in attendance at each poster during the entire presentation time. • Do not allow yourself to be monopolized for an inordinate period of time by a single individual. • Please remove your materials from the poster board immediately after the session. Materials left on the boards after the session will be discarded. • The only handouts allowed in the Poster Hall include exact copies of your poster or business cards; all other types of handouts are not allowed in the Poster Hall.

Impact Is the poster eye-catching? • TitleShort, punchy, informative? True reflection of content? • Authors Is it clear who is the presenter? Is there "Corporate Visual Identity"? • Layout Is it tidy? Is it easy to follow the sequence? • Readability Are print size, font etc. appropriate? • Purpose Introduction useful? Are Aims/Objectives clearly stated? • Methods Are these appropriately presented? • Results Have table, graphs and figures been used well? Is it easy for the reader to understand data? • Conclusions How are they presented? Do they match aims? • Practicalities Think about shape, size, portability, fixing method, positioning Look at posters critically and consider one of these elements:

What is an e-Poster? • The e-poster is a ppt presentation accessible on a computer during the congress • The computer stands will be located at the exhibition area or poster area • The e-poster provide a user-friendly browsing engine available to all congress attendees during the congress meeting • The e-poster system allows you to search presentations for authors name and abstract title • The e-poster allows you to save chosen presentation once on site either on your own USB flash drive or send it by e-mail.

Final Points … • Why are you doing a poster? • to communicate results • to advertise your work, yourself, your department • Be proactive - • identify yourself • (photo on poster, presenter badge, add poster number to badge) • Be remembered - • first name on poster, email address, take-away handouts, reprints

Woolston C. 2016. Conference presentations: Lead the poster parade. Nature 536: 115-117. http://dx.doi.org/10.1038/nj7614-115a

Poster Presentation