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Photosynthesis. Energy & Life. Overview of Photosynthesis. Autotrophs. Plants and some other types of organisms that contain chlorophyll are able to use light energy from the sun to produce food. Autotrophs. Autotrophs include organisms that make their own food
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Photosynthesis Energy & Life
Autotrophs Plants and some other types of organisms that contain chlorophyll are able to use light energy from the sun to produce food.
Autotrophs • Autotrophs include organismsthat make their own food • Autotrophs can use the sun’s energy directly Euglena
Heterotrophs • Heterotrophs are organisms that can NOT make their own food • Heterotrophs can NOT directly use the sun’s energy
Energy • Energy Takes Many Forms such as light, heat, electrical, chemical, mechanical • Energy can be changed from one form to another • Energy can be stored in chemical bonds & then released later Candles release energy as HEAT & LIGHT
ATP – Cellular Energy • Adenosine Triphosphate • Contains two, high-energy phosphate bonds • Also contains the nitrogen base adenine & a ribose sugar
ADP • Adenosine Diphosphate • ATP releases energy, a free phosphate, & ADP when cells take energy from ATP One phosphate bond has been removed
Sugar in ADP & ATP • Called ribose • Pentose sugar • Also found on RNA
Importance of ATP Principal Compound Used To Store Energy In Living Organisms
Releasing Energy From ATP • ATP is constantly being used and remade by cells • ATP provides all of the energy for cell activities • The high energy phosphate bonds can be BROKEN to release energy • The process of releasing ATP’s energy & reforming the molecule is called phosphorylation
Releasing Energy From ATP • Adding A Phosphate Group To ADP stores Energy in ATP • Removing A Phosphate Group From ATP Releases Energy & forms ADP Loose Gain
Cells Using Biochemical Energy Cells Use ATP For: • Active transport • Movement • Photosynthesis • Protein Synthesis • Cellular respiration • All other cellular reactions
More on ATP • Cells Have Enough ATP To Last For A Few Seconds • ATP must constantly be made • ATP Transfers Energy Very Well • ATP Is NOT Good At Energy Storage
Glucose • Glucose is a monosaccharide • C6H12O6 • One Molecule of glucose Stores 90 Times More Chemical Energy Than One Molecule of ATP
Photosynthesis • Involves the Use Of light Energy to convert Water (H20) and Carbon Dioxide (CO2) into Oxygen (O2) and High Energy Carbohydrates (sugars, e.g. Glucose) & Starches
Investigating Photosynthesis • Many Scientists Have Contributed To Understanding Photosynthesis • Early Research Focused On The Overall Process • Later Researchers Investigated The Detailed Chemical Pathways
Early Questions on Plants Several Centuries Ago, The Question Was:Does the increase in mass of a plant come from the air? The soil? The Water?
Van Helmont’s Experiment 1643 • Planted a seed into A pre-measured amount of soil and watered for 5 years • Weighed Plant & Soil. Plant Was 75 kg, Soil The Same. • Concluded Mass Came From Water
Priestley’s Experiment 1771 • Burned Candle In Bell Jar Until It Went Out. • Placed Sprig Of Mint In Bell Jar For A Few Days. • Candle Could Be Relit And Burn. • Concluded Plants Released Substance (O2) Necessary For burning.
Ingenhousz’s Experiment 1779 Repeated Priestly experiment with & without sunlight
Results of Ingenhousz’s Experiment • Showed That Priestley’s Results Only Occurred In The Presence Of Sunlight. • Light Was Necessary For Plants To Produce The “Burning Gas” or oxygen
Julius Robert Mayer 1845 Proposed That Plants can Convert Light Energy Into Chemical Energy
Samuel Ruben & Martin Kamen1941 Used Isotopes To Determine That The Oxygen Liberated In Photosynthesis Comes From Water RUBIN KAMEN
Melvin Calvin 1948 • First to trace the path that carbon (CO2) takes in forming Glucose • Does NOT require sunlight • Called the Calvin Cycle or Light Independent Reaction • Also knownas the Dark Reaction
Rudolph Marcus 1992 • Studied the Light Independent Reactions • First to describe the Electron transport Chain
Pigments • In addition to water, carbon dioxide, and light energy, photosynthesis requires Pigments • Chlorophyll is the primary light-absorbing pigment in autotrophs • Chlorophyll is found inside chloroplasts in the thylakoids
Light and Pigments • Energy From The Sun Enters Earth’s Biosphere As Photons • Photon = Light Energy Unit • Light Contains A Mixture Of Wavelengths • Different Wavelengths Have Different Colors
Light & Pigments • Different pigments absorb different wavelengths of light • Photons of light “excite” electrons in the plant’s pigments • Excited electrons carry the absorbed energy • Excited electrons move to HIGHER energy levels
Pigments There are 4 main types of pigment molecules: Chlorophyll a Chlorophyll b Xanthophyll Carotenes
Chlorophyll a • Found in all plants, algae, & cyanobacteria • Makes photosynthesis possible • Participates directly in the Light Reactions • Can accept energy from chlorophyll b
Chlorophyll b • Chlorophyll b is an accessory pigment • Chlorophyll b acts indirectly in photosynthesis by transferring the light it absorbs to chlorophyll a • Like chlorophyll a, it absorbs red & blue light and REFLECTS GREEN
Objectives • Summarize how energy is captured from sunlight in the first stage of photosynthesis. • Analyze the function of electron transport chains in the second stage of photosynthesis. • Relate the Calvin cycle to carbon dioxide fixation in the third stage of photosynthesis. • Identify three environmental factors that affect the rate of photosynthesis.
Pigment Chlorophyll Carotenoid Thylakoid Electron Transport Chain NADPH Carbon Dioxide Fixation Calvin Cycle Key Terms
Chloroplast Mesophyll Cell Stoma Where does Photosynthesis occur? • Plants/Autotrophs – produce their own food (glucose) • Process called photosynthesis • Mainly occurs in the leaves: a. stoma - pores b. mesophyll cells-chloroplasts found in this area
Structure of the Chloroplast • Double membrane organelle • Outer membrane smooth • Inner membrane forms stacks of connected sacs called thylakoids • Thylakoid stack is called the granun (grana-plural) • Gel-like material around grana called stroma
The 2 Stages of Photosynthesis • Stage 1-Light Reaction • Energy is captured from sunlight by chlorophyll and other pigments present. • Light energy is converted to chemical energy as water is split, which is temporarily stored in ATP & the energy carrier molecule NADPH and O2 gas is given off • Stage 2 -Light Independent Reaction/Dark Reaction/Calvin Cycle • The chemical energy stored in ATP & NADPH powers the formation of organic compounds from the combination of RuBP and the CO2 to eventually makes Glucose.
Stages of Photosynthesis RuBP Celluslose
The Photosynthesis Equation Light 6CO2 + 6H20 C6H12O6 + 6O2 Carbon Dioxide Water Sugars Oxygen (High Energy)