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Tomimaru Y, et al. Supplementary Figure 1A. 6.0. 4.0. Relative miRNA-21 expression ( D Ct) (normalized). 2.0. 0. -2.0. I (n=67). II (n=16). IIIA (n=43). 0 (n=52). 1 (n=37). 2 (n=23). 3- (n=14). 0 (n=11). 1 (n=62). 2 (n=27). 3- (n=26). A (n=58). B (n=37). C

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  1. Tomimaru Y, et al. Supplementary Figure 1A 6.0 4.0 Relative miRNA-21 expression (DCt) (normalized) 2.0 0 -2.0 I (n=67) II (n=16) IIIA (n=43) 0 (n=52) 1 (n=37) 2 (n=23) 3- (n=14) 0 (n=11) 1 (n=62) 2 (n=27) 3- (n=26) A (n=58) B (n=37) C (n=31) TNM staging CLIP scoring JIS scoring BCLC staging

  2. Tomimaru Y, et al. Supplementary Figure 1B 6.0 4.0 Relative miRNA-21 expression (DCt) (normalized) 2.0 0 -2.0 B-C- (n=11) B+C- (n=62) B-C+ (n=27) B+C+ (n=26) cirrhosis (-) (n=67) cirrhosis (+) (n=59) Liver cirrhosis Viral status

  3. Tomimaru Y, et al. Supplementary Figure 2A AFP miRNA-21 AFP + miRNA-21 100 100 100 80 80 80 60 60 60 Sensitivity (%) Sensitivity (%) Sensitivity (%) 40 40 40 AFP + miRNA-21 AFP miRNA-21 AUC 0.743 AUC 0.755 AUC 0.720 20 20 20 20 40 60 80 20 40 60 80 20 40 60 80 0 100 0 100 0 100 100-Specificity (%) 100-Specificity (%) 100-Specificity (%)

  4. Tomimaru Y, et al. Supplementary Figure 2B AFP miRNA-21 AFP + miRNA-21 100 100 100 80 80 80 60 60 60 Sensitivity (%) Sensitivity (%) Sensitivity (%) 40 40 40 AFP + miRNA-21 AFP miRNA-21 AUC 0.886 AUC 0.928 AUC 0.869 20 20 20 20 40 60 80 20 40 60 80 20 40 60 80 0 100 0 100 0 100 100-Specificity (%) 100-Specificity (%) 100-Specificity (%)

  5. Tomimaru Y, et al. Supplementary Figure 3 100 80 Low tumoral miRNA-21 group (n=34) 60 * Cumulative survival rate without recurrence (%) 40 * 20 High tumoral miRNA-21 group (n=92) 0 0 1 2 3 4 5 6 7 Time after surgery (years)

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