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Expression of CEACAM1 and Apc in CT51 colon carcinoma cells and Apc 1638/+ or Ceacam1 mice.

C. G3PDH expression. APC. CEACAM1. Neg control. -/-. +/-. +/-. +/+. +Neo. +/+. ladder. WT. +CC1L. bp. 400. 300. 200. 100. Figure S1. Expression of CEACAM1 and Apc in CT51 colon carcinoma cells and Apc 1638/+ or Ceacam1 mice. B. A. APC expression. CEACAM1 expression.

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Expression of CEACAM1 and Apc in CT51 colon carcinoma cells and Apc 1638/+ or Ceacam1 mice.

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  1. C G3PDH expression APC CEACAM1 Neg control -/- +/- +/- +/+ +Neo +/+ ladder WT +CC1L bp 400 300 200 100 Figure S1 Expression of CEACAM1 and Apc in CT51 colon carcinoma cells and Apc1638/+ or Ceacam1 mice. B A APC expression CEACAM1 expression Neg control Neg control +Neo WT +CC1L +Neo +/+ +CC1L +/- ladder ladder WT +/+ +/- -/- bp bp 300 400 200 300 100 200 100 Figure Legend: Total RNA was prepared from CT51 cells (WT) and those transfected with the empty vector (+Neo) or with the Ceacam1-L cDNA (+CC1L) or mouse intestines from either Apc1638N (B and C)or Ceacam1 (A and C) mice. The RNA was subjected to reverse transcription and PCR amplification according to protocols described in Materials and Methods and the resulting DNA fragments were separated on agarose gels. Expression of CEACAM1 was revealed by the presence of a 270 bp fragment whereas APC expression produced a 120 bp frament. The integrity of the samples was controlled by gauging expression of the glyceraldehyde 3 phosphate dehydrogenase RNA (G3PDH, 210 bp).

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