Multiple Sclerosis patients treated with Apitherapy: Immunological pattern

1. بسم الله الرحمن الرحيم Multiple Sclerosis patients treated with Apitherapy: Immunological pattern Ahmad G. Hegazi, Khaled Al-Menabbawy, Mona Ganem, Samira Shawky, Eman H. Abdel-Rahman and Suzette I. Helal, National Research Center, Dokki, Giza, Egypt ahmed@ahmedhegazi com and ahmedhegazi128@gmail.com www.ahmedhegazi.com

2. Multiple Sclerosis patients treated with Apitherapy: Immunological pattern Novel treatment of localized plaque psoriasis by some bee products • Special thanks to Scientific Committee of the THE 2nd INTERNATIONAL CONFERENCE ON QUALITY MEDICATION IN PROPHETIC MEDICINE King Abdulaziz University April 5-7, 2016 Jeddah, KSA

5. Definition: • Multiple Sclerosis ( MS ): Is a chronic disease characterized by • multiple areas of central nervous system ( CNS ) • white matter inflammation • demyelination , • sclerosis • it usually begins in young adults.

6. Multiple Sclerosis ( MS ): Also known as disseminated sclerosis or encephalomyelitis disseminata). It is a disease in which the fattymyelinsheaths around the axons of the brain and spinal cord are damaged, leading to demyelination and scarring as well as a broad spectrum of signs and symptoms

7. onset usually occurs in young adults, it is more common in females. It has a prevalence that ranges between 2 and 150 per 100,000. Multiple Sclerosis Society standardized four subtype definitions:

8. relapsing remitting, • secondary progressive, • primary progressive, • progressive relapsing.

9. Signs & Symptoms : • The person with MS can suffer almost any neurological symptom or sign, including: • changes in sensation, • muscle weakness, • difficulty in moving; • difficulties with coordination and balance • spasm,

10. Signs & Symptoms : • problems in speech or swallowing, • visual problems, • fatigue, • pain • bladder and bowel difficulties. • Cognitive impairment of varying degrees • emotional symptoms of depression • or unstable mood are also common.

11. Causes Of MS : • Most likely MS occurs as a result • Genetics: • Environmental factors • Infections

12. Radiological Findings

16. Materials & Methods

17. Ninety patients with MS, • ages ranged between 26-71 years, • subjected to complete clinical and neurological history and examination to confirm the diagnosis.

18. All cases were under their regular treatment either by corticosteroids, or interferon. • These cases were divided into two main groups, each group consists of 45 cases (15 males and 30 females)

19. Group I received • honey, pollen, royal jelly and propolis • treated with bee acupuncture 3 times weekly, for 12 months,

20. started gradually by one sting then gradually increase up to 25 stings per session, in addition to their medical treatment

21. Apiacupuncture was done by bee stings for regulating the immune system.

22. Bee acupuncture points

23. Du 14

24. Du 13

25. LI11

26. SP6

27. SP 9

28. ST 36

29. points for MS Pat Wagner Buttocks Jiagi points • for cervical area and lumber area • vision points

30. GB2 Auditory Convergence GB2

31. LIV3

32. Group II • remains on their ordinary medical treatment only.

33. All cases were subjected to • complete clinical • neurological history • examination to confirm the diagnosis.

34. All patients were instructed to receive • 2000-3000 mg Vitamin C, • 15 mg Vit. B1, • 3 mg Vit. B2, • 2mg Vit. B6, • 5 mg Vit. B12,

35. 25 mg Folic acid, • 3 mg Calcium pantothenate, • 15mg Nicotinamide, • 20 mg L- Arginine, • 20 mg L- Lysine • and 3 mcg Biotin / day.

36. Serum samples were obtained from patients with clinically definite MS for estimation of  serum levels of immunoglobulin E (Hirano et al., 1989) • using commercially available ELISA kits according to the manufacturers' directions.

37. Serum cytokine levels • . IFN-γ, • interleukin (IL) 1β, • IL-4, • IL-6, • IL-10, • tumor necrosis factor alpha (TNFα) were detected.

38. Serum cytokine levels were assessed using enzyme inked ‑ immunosorbent assays (Abrams, 1995) using commercially available ELISA kits according to the manufacturers' directions (kits produced by Bender Med System, Vienna, Austria).

39. All these investigations were done at the beginning of the study and by the end of one year of supplementation and bee sting sessions

40. Results

41. We found that 8 patients out of 12 (paraparesis cases), • showed some improvement regarding • their defects in gait, • bowel control, • constipation • and urination,

42. while 12 cases out of 16 cases ( quadriparesis cases), • showed some mild improvement in their • movement in bed, • and better improvement in bed sores, • sensation, • and better motor power, • only two cases of them ( 12.5% ) were able to stand for few minutes with support.

43. Symptoms Score Improvement Recorded Every Two Months (1-12):

44. Symptoms Score Improvement Recorded Every Two Months (1-12):

45. Symptoms Score Improvement Recorded Every Two Months (1-12):

46. Symptoms Score Improvement Recorded Every Two Months (1-12):

47. Symptoms Score Improvement Recorded Every Two Months (1-12):

48. Symptoms Score Improvement Recorded Every Two Months (1-12):

49. Symptoms Score Improvement Recorded Every Two Months (1-12):

50. Symptoms Score Improvement Recorded Every Two Months (1-12):