1 / 17

PBIO 4500/5500: Biotechnology and Genetic Engineering

PBIO 4500/5500: Biotechnology and Genetic Engineering. Welcome! Complete the student information form. Tell the class about you. All the course information is on the course web site; I do not use Blackboard. Let’s go over the syllabus. Get the 5 th Edition of Molecular Biotechnology.

rnixon
Download Presentation

PBIO 4500/5500: Biotechnology and Genetic Engineering

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. PBIO 4500/5500:Biotechnology and Genetic Engineering Welcome! Complete the student information form. Tell the class about you. All the course information is on the course web site; I do not use Blackboard. Let’s go over the syllabus. Get the 5th Edition of Molecular Biotechnology. Any questions?

  2. Chapter 1 - The Development of Molecular Biotechnology Molecular Biology/Biotechnology terminology Milestones in molecular biotechnology Model organisms for molecular/cellular biology Transcription and translation Prokaryotic gene organization & expression

  3. Terminology • Molecular biology-the study of biology on a molecular level including the structure, function, and makeup of biologically important molecules such as DNA, RNA, and proteins • Recombinant DNA technology-a set of techniques for manipulating DNA, including: the identification and cloning of genes; the study of the expression of cloned genes; and the production of large quantities of gene product • Genetic engineering-the process of transferring DNA from one organism into another that results in a genetic modification • Biotechnology-production of goods and services using biological organisms, systems, and processes • Molecular biotechnology-rDNA technology + biotechnology

  4. Table 1.1

  5. Table 1.1 (Continued)

  6. Many scientific disciplines contribute to molecular biotechnology, which generates a wide range of commercial products. Figure 1.1

  7. Model Systems for Molecular/Cellular Biologyhttps://www.dnalc.org/resources/animations/model_organisms.html • Viruses • Bacteria (E. coli) • Yeast (Saccharomyces cerevisiae) • Round worms (Caenorhabditis elegans) • Planarian (Schmidtea mediterranea) • Alga (Chlamydomonas reinhardtii) • Fruit fly (Drosophila melanogaster) • Zebrafish (Danio rerio) • Arabidopsis thaliana (thale cress) • Mouse (Mus musculus)

  8. Figure 1.13 Each model experimental organism used in molecular and cell biology has advantages for certain types of studies.

  9. Central Dogma of Biology transcription translation $$$ DNA RNA Protein reverse transcription Molecular Biotechnology DNA replication

  10. Chemical structure of DNA & RNA

  11. Chemical structure of dsDNA

  12. Chemical structure of dsDNA

  13. Prokaryotic Gene Structure and Expression

  14. In prokaryotes, RNA polymerase binds to the -10 and -35 regions of the promoter relative to the start site of transcription (+1) promoter operator

  15. Prokaryotic Gene Structure Eukaryotic Gene Structure Enhancers/ silencers

More Related