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Genetic monitoring methodologies for allogamous crop wild relatives

Genetic monitoring methodologies for allogamous crop wild relatives. Group B - results. Sampling. Annual – every year Perennials – as often (generation time). Risk of loss of diversity. Measuring loss of diversity Morphological characters evolution Molecular tools for evolution.

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Genetic monitoring methodologies for allogamous crop wild relatives

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  1. Genetic monitoring methodologies for allogamous crop wild relatives Group B - results

  2. Sampling • Annual – every year • Perennials – as often (generation time)

  3. Risk of loss of diversity • Measuring loss of diversity • Morphological characters evolution • Molecular tools for evolution

  4. Loss genetic diversity within population decreasing viability extinction • Losing interesting variation within populations

  5. For the study of evolution in genetic drift, need to use genetic diversity indexes (indicators of overall diversity) Or • To use tools for specific useful traits to monitor over time

  6. 3 kinds of markers levels • Diversity • Gene marker or processes • Fitness (related to specific traits) • Demographic studies • Related to fecundity Differentiate between genetic and environmental stochasticity

  7. Knowledge of heritability molecular characters – use it as surrogate of genetic diversity • Use list of descriptors as a base for cwr characterisation

  8. Ex situ / in situ • Link • Coordination • Ex situ bottleneck in characterisation/evaluation, useful for in situ

  9. Spatial structure (key point) • Distribution of individuals (further inbreeding) • Spatial structure, diversity on morphological characters • Genetic neighbourhood • Implication in restoration

  10. Number of sample/periodicity • Higher and more frequent than for autogamous • How to sample • Allogamous – Random / stratified • Vegetatively propagated – • If knowledge available – random • If knowledge is not available – random / stratified

  11. RECOMMENDATIONS • Genetic reserve should have a backup ex situ collection • In situ/ex situ characterisation • To promote use • Carry out in depth studies on cwr • Useful information on genetic knowledge

  12. Reinforce links with ex situ conservation • Documentation • Improve ecogeographic survey • Development of primers • Sources • Genebanks • Breeding institutes • Universities • CG centres • At the National level – set priorities for developing primers

  13. Transferability of primers are not easy • Policy issues, germplasm exchange required • Take into consideration any international agreements, conventions, treaties,…

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