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RECIPE Charquemont Progress meeting 23 - 26/10/2003

RECIPE Charquemont Progress meeting 23 - 26/10/2003. UK progress Rebekka Artz Stephen Chapman Colin Campbell. I. WP01 UK Field site. Middlemuir Moss, between Strichen and New Pitsligo, NE Scotland. Bare milled peat (0-5 yrs). 1 st succession peat (> 5yrs). Regenerating peat (> 50 yrs).

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RECIPE Charquemont Progress meeting 23 - 26/10/2003

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  1. RECIPE Charquemont Progress meeting23 - 26/10/2003 UK progress Rebekka Artz Stephen Chapman Colin Campbell

  2. I. WP01 UK Field site • Middlemuir Moss, between Strichen and New Pitsligo, NE Scotland

  3. Bare milled peat (0-5 yrs)

  4. 1st succession peat (> 5yrs)

  5. Regenerating peat (> 50 yrs)

  6. > 50 yrs 5 -10 yrs Bare

  7. Table 1. Approximate species distribution at RECIPE WP01 field sites Key: EV – Eriophorum vaginatum, EA – Eriophorum angustifolium, SF – Sphagnum fallax, SM – Sphagnum magellanicum, SC – Sphagnum capillifolium, SCU – Sphagnum cuspidatum, CI – Campylopus introflexus, PC – Polytrichum commune, HS – Hypnum sp. (jutlandicum or cypressiforme), PS – Pleurozium schreberi, CS – Calluna sp., O – others (grasses etc.)

  8. II. Field site preliminary data

  9. Water table in plots

  10. Field measurements of CO2 and CH4 fluxes Methane added

  11. Bare peat – CO2 - 90 cm - 41 cm

  12. Bare peat – CH4 - 90 cm - 41 cm

  13. - 7 cm - 7 cm 1st succession peat – CO2 - 22 cm - 22 cm

  14. - 7 cm - 7 cm 1st succession peat – CH4 - 22 cm - 22 cm

  15. Regenerating peat – CO2 - 38 cm - 38 cm

  16. Regenerating peat – CH4 - 38 cm - 38 cm

  17. III. Fungal community structure analysisby DGGE of ribosomal 18S and/or ITS sequencesPreliminary data

  18. * p < 0.05 Test DNA extractions for shipping methods * * *

  19. Sphagnum Low humification M + NT/SLN/S FD/S NT/P1LN/P1 FD/P1

  20. Medium humification High humification + M NT/P2LN/P2 FD/P2 NT/ELN/E FD/E + M

  21. PCR FD/P2 Milled surface M + P2oldP2new NT/MLN/M FD/M + + M

  22. Regenerating S E M + NT LN FD

  23. but: GELS CANNOT BE INTERCOMPARED (YET) Fungal DGGE analysis shows significant differences in band patterns according to peat depth

  24. IV. Community level physiological profilingby MicroRespPreliminary data

  25. ‘Standard’ method utilises sieved soil of ca. 40% WHC problematic with peat due to irreversible drying and fibrosity • Protocol adapted to use slurry (peat blended with water 1:10) • tested with glucose (150 and 75 mM)

  26. 150 mM glucose, 2.5 mM bicarb • only the surface sample at Regenerating peat site gives • significantly higher SIR than a water blank (not sterile). No distinction possible between samples so far.

  27. 75 mM glucose, varying bicarb

  28. All 4 peat depths at Regenerating site, 0-5 cm horizon at 1st succession site, and all except the 0-5 cm horizon at the bare site show significantly higher CO2 evolution than the (water only) control. Different samples show different CO2 curves.

  29. Lowering the buffering capacity of the detection system allows for sample distinction. • Further work: • incorporation of different carbon sources to allow for CLPP using 32 carbon sources per plate (n = 3) or 16 carbon sources (n = 6). • Anoxic system?

  30. V. RECIPE WEBSITE Preliminary version available at http://www.macaulay.ac.uk/recipe Will go public soon …

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