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Chemistry in action…. Or…. Ask your friendly chemist…

Chemistry in action…. Or…. Ask your friendly chemist…. Chemistry of electrophoresis and electrolysis Electric fields and electric currents.

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Chemistry in action…. Or…. Ask your friendly chemist…

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  1. Chemistry in action….Or….Ask your friendly chemist…

  2. Chemistry of electrophoresis and electrolysisElectric fieldsand electric currents

  3. SDS-PAGE, DNA electrophoresis and the need for gel (sieving medium)Without a viscous medium, all move at ~the same rate in electric fieldGel structure serves to retard larger solute particlesDNA molecules, SDS proteins have ~equal charge/massElectrophoresis occurs between the electrodes (field-driven).

  4. Electrolysis always occurs during electro-phoresis.Cathode produces H2 at twice the rate that anode produces O2Current is carried by solute ions. Electrons aren’t soluble in H2O.Example: TAE buffer; tris suppliescations(+),acetatesupplies anions(-).Electrolysis occursat the electrodes.

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