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Introgression mapping in the grasses Lolium perenne and Festuca pratensis

Introgression mapping in the grasses Lolium perenne and Festuca pratensis. Julie King. LpLpLpLp x FpFp (28) (14). LpLpFp x LpLp (21) (14). BC 1 (14). Production of substitution lines. Chromosome 1. Chromosome 5.

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Introgression mapping in the grasses Lolium perenne and Festuca pratensis

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  1. Introgression mapping in the grasses Lolium perenne and Festuca pratensis Julie King

  2. LpLpLpLp x FpFp (28) (14) LpLpFp x LpLp (21) (14) BC1 (14) Production of substitution lines

  3. Chromosome 1 Chromosome 5 Chromosome 2 Chromosome 6 Chromosome 3 Chromosome 7 Chromosome 4

  4. LpLpLpLp x FpFp (28) (14) LpLpFp x LpLp (21) (14) LpLp(Fp) x LpLp (13Lp+1Fp) (14) BC1 LpLp(Fp) (14) BC2 BC2 introgression population

  5. Lolium/Festuca substitution lines Lolium 2n=2x=14 3 5 6 1 2 4 7 7 Lolium/Festuca substitutions 5 2 3 6 1 4 7

  6. Introgression chromosome series X

  7. Genetic map of chromosome 3

  8. BC2 plants used for physical mapping 18 11 3/26 3 3/10 17 56 83 2/3 3/23 19 99 3/2 92 36 6

  9. Genetic Distance Physical distance cM % 0 0 Genotype H37/M36(2),H37/M36(3), E32/M40(3), . H43/M40(5), H43/M40(3),H36/M36(2), 7.9 7.1  18 H44/M39(5), E32/M40(4) E33/M41(1), 14.8 11.4  11 H42/M35(1). 25 12.4 H36/M36(3),H35/M39(2), H38/M36(2), E32/M41(2),E38/M40(3), H32/M37(2), E36/M37(3). 3/26  H35/M42(1), E33/M37(3). 27.8 15.5 6  H38/M36(1),H35/M39(3), E34/M35(1), 17.9 32.7  3 H42/M35(2),H44/M39(1), E34/M33(2),. 19.7  3/10 34 H36/M37(3),H37/M33(2),H32/M37(1), H35/M42(3), E32/M41(1), E36/M43(3), H37/M36(1). 28.9 37.6  36 H41/M33(2),H43/M40(2),H36/M37(2),H39/M37(2), E38/M40(2),E38/M40(4), H31M40(1),H37/33(1), H43/M40(4), H36/M36(1) E33/M38(1),E36/M37(1), H43/M40(6), 17 56.0  38.0 H35/M41(2), E33/M37(7),E33/M37(6), H36/M33(2). 44.5  59.2 83 H35/M39(4), 47.3 59.2 92 Centromere 49.2 E39/M38(1),E40/M40(1), H33/M33(1),H35/M39(5), H38/M43(1),H44/M39(4),H44/M39(3), E36/M43(1) H41/M33(1),H33/M38(2),H39/M37(1), NOR 56.6 62.5  61.2 3/2 E38/M40(1),E32/M41(3),E40/M40(2), H43/M40(1), E36/46(4),E36/M46(1),E36/M43(4),E36/M37(5), E36/M37(2),E34/M33(1),E33/M41(2). 68.5  61.9 99 70.0 NOR H42/M35(3), E34/M32(2),E33/M37(5). 73.1 61.9  83 E39/M38(2),E40/M40(3), E38/M32(2), H35/M41(1), E39/M32(1),E39/M32(2), H35/M39(1). 64.2 80.0  19 66.2 82.0  E38/M32(1),E36/M46(3),E39/M32(3),E33/M38(2), 56 68.9 E33/M37(4). 82.4 3/23  H44/M39(2),H35/M42(2), E36/M43(2),E36/M46(2),E33/M37(2), 86.6 73.9 2/3 E36/M37(6),E34/M32(3),E33/M41(3), H31/M40(2),  E39/M32(4), H33/M33(2),H37/M33(3),H36/M37(1), E39/M38(3), H33/M38(1),H42/M35(4),H36/M33(1), 80.9 100

  10. Genetic Distance Physical distance cM % 0 0 Genotype 7.9 7.1  18 14.8 11.4  11 25 12.4 3/26  27.8 15.5 6  17.9 32.7  3 19.7  3/10 34 28.9 37.6  36 17 56.0  38.0 44.5  59.2 83 47.3 59.2 92 Centromere 49.2 NOR 56.6 62.5  61.2 3/2 68.5  61.9 99 70.0 NOR 73.1 61.9  83 64.2 80.0  19 66.2 82.0  56 68.9 82.4 3/23  86.6 73.9 2/3  80.9 100 RZ783, C112 C949 CDO345 C250, C122, WG889, BCD128 CDO455 R1928, PSR370, RZ382, PSR394, CDO920 R1613 CDO328 CDO466, C30

  11. Rice chromosome 1: BAC No.BAC/PAC BAC/PAC coordinates 1 P0672D08 1 – 173,729 3 P0005A05 300,777 – 436,798 8 P0463F06 851,331 – 919,450 14 P0037C04 1,345,101 – 1,437,630 16 P0408F06 1,566,828 – 1,698,033 22 P0024G09 2,381,624 – 2,444,317 25 P0009G03 2,715,057 – 2,855,651 …

  12. BAC 227 (PAC clone PO686E09) Festuca AATTCTGGTTCATGTGTTCCTGTCGACATTGAGTGGCCTCCTAGAAGAGCA Lolium (4n) AATTCTGGTTCATGTGTTCCTGTCGACATTGAGTGGCCTCCTAGAAGAGCA Lolium (2n) AATTCTGGTTCATGTGTTCCTGTCGACATTGAGTGGCCTCCTAGAAGAGCA CATCATCTGAACTTTAATGCACCATTTTCTATGAACATGGATACTAAATCTG CATCATCTGAACTTTAATGCACCATTTTCTATGAACATGGATACTAAATCTG CATCATCTGAACTTTAATGCACCATTTTCTATGAACATGGATACTAAATCTG GAGAGCTGTCAAATGACGGACTCAATGATGTCGATCCTTTTGCCGATTGGC GAGAGCTGTCAAATGACGGACTCAATGATGTTGATCCTTTTGCCGATTGGC GAGAGCTGTCAAATGACGGACTCAATGATGTTGATCCTTTTGCCGATTGGC

  13. BAC mapping discrepancies BAC Subline 167 7 170 5 174 4 178 2 261 7 326 3 + 5

  14. Genetic Distance Physical distance cM % Genotype 0 0 , 9% BACs 7.9 7.1  18 15.5% 14.8 11.4  11 25 12.4 3/26  27.8 15.5 6  17.9 32.7  3  3/10 34 19.7 28.9 37.6  36 . 17 56.0  38.0 44.5  59.2 83 82% BACs 47.3 59.2 92 64.5% Centromere 49.2 NOR 56.6 62.5  61.2 3/2 68.5  61.9 99 70.0 NOR 73.1 61.9  83 80.0 64.2  19 66.2 82.0  56 68.9 82.4 3/23  9% BACs 86.6 73.9 2/3  20% 80.9 100

  15. Genetic Distance Physical distance cM % Genotype 0 0 , 7.9 7.1  18 14.8 11.4  11 25 12.4 3/26  27.8 15.5 6  17.9 32.7  3  3/10 34 19.7 28.9 37.6  36 35% recombination = very low 50% BACs 47% genes 53% = T + H . 17 56.0  38.0 44.5  59.2 83 47.3 59.2 92 Centromere 49.2 NOR 56.6 62.5  61.2 3/2 68.5  61.9 99 70.0 NOR 73.1 61.9  83 80.0 64.2  19 66.2 82.0  56 68.9 82.4 3/23  86.6 73.9 2/3  80.9 100

  16. Determining genetic control of target traits • Locate gene(s) controlling trait to chromosome(s) using substitution lines. • Locate gene(s) controlling trait to a particular bin using physical mapping population. • Use bin mapped rice BACs to exploit rice genome for further markers /candidate genes. • Springboard for gene isolation.

  17. Nutrient use efficiency High nitrogen uptake - chr. 3 High nitrogen utilisation - chr. 4

  18. All involved: Ian King Ian Armstead Iain Donnison Luned Roberts John Harper Kirsten Skot Lin Huang

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