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High Throughput Crystallography - Instrumentation Barbara McIntosh

High Throughput Crystallography - Instrumentation Barbara McIntosh Global Product Mgr – Cartesian Dispensing. Hierarchy. synQUAD Technology. Ink jet mechanism for fluid delivery Non Contact Nanoliter to microliter volume range Independent Channel Control Patented granted in 1998.

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High Throughput Crystallography - Instrumentation Barbara McIntosh

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  1. High Throughput Crystallography - Instrumentation Barbara McIntosh Global Product Mgr – Cartesian Dispensing

  2. Hierarchy synQUAD Technology

  3. Ink jet mechanism for fluid delivery Non Contact Nanoliter to microliter volume range Independent Channel Control Patented granted in 1998 synQUAD™ Technology Non Contact Dispensing

  4. master controller synQUAD valve stage stepper motor syringe stepper motor synQUAD™ Non Contact Dispensing • Valve, Syringe, and Stage are synchronized

  5. Characteristics of synQUAD Technology • Wide dispense range • 20 nanoliter to 20 microliter • Excellent linearity • Precise and accurate • Reproducibility less than 10%CV • Precision less than  5% • Non-contact dispense mechanism • Easier mechanical alignment (384, 1536,…) • No washing of tips for bulk dispense • Multiple liquid handling modes • Aspirate/Dispense • Continuous Reagent Dispensing

  6. Aspirate and Dispense u v

  7. Continuous Reagent Dispensing u v R e a g e n t R e s e r v o i r

  8. Features for Protein Crystallization • Speed • Fill 1536 in under 60 seconds • Flexibility • Aspirate/Dispense or Bulk Dispense • Multiple reagents simultaneously • Proteins, cells, particles • Mixing • Fluidic inertia causes efficient reagent mixing • Precision • <3% CV at 1 uL, <5% CV at 100 nL

  9. synQUAD Video

  10. Overview Oil is added to each well Precipitant solutions kept in deep well plates Dispenser transfers drops to crystallization wells Dispenser Aspirate/Dispenses protein per well “Suspended Drop” - Non Contact Dispensing Directly into Oil (patent pending) Microbatch Process

  11. Advantages of synQUAD for Microbatch Non contact Dispense through the parafin oil Fluidic inertia High Speed Microbatch Process

  12. Microbatch Products • PixSys 4200 • 8-16 Channel synQUAD • 10 Plate Position Deck • Extended Head Options • Humidity Controlled Environment Option

  13. Microbatch “Suspended Drop”

  14. Overview Add Mother Liquors to 96 well plate Aspirate Precipitant to Sitting Shelf Aspirate Protein & Dispense to Sitting Shelf Protein Chilled Source Plate or vial Cover Plate Pressure adhesive Seal Process less than 20 seconds Sitting Drop

  15. Advantages of synQUAD for Sitting Drop Mechanical alignment Non contact Fast No Wash Step required Sitting Drop Process

  16. Sitting Drop Products • ProSys 4210 • Overhead Gantry • Minimum 8 channel Dispense • 250 mm/sec • 18 Plate Position Deck • Humidity Controlled Environment

  17. New Products - Protein Crystallography

  18. New Products Honeybee Protein Crystallization Systems • Advantages • Low volume Dispensing of PEGs • Speed • Flexible • Multiple Techniques for PC • Other Applications

  19. New Products Cartesian Personal Pipettor 96 Plungers Air Displacement Technology 100 – 550 uL Non Contact

  20. New Products Honeybee Protein Crystallization Systems • 2 Models • 4 Configurations • Benchtop – Passive Nest – 8+1 • Benchtop – Active Deck – 8+1 • Benchtop – Active Deck – 16 + 1 • Automated Gantry – 16 + 1

  21. New Products Honeybee Protein Crystallization Systems • Benchtop Descriptions • Nest • Based on PixSys platform • Actuated Plate Positions • Dual Wash Station • Protein Vial Holders • Extra Passive Plate Positions • Humidity Chamber • Digital Readouts • Dispense Head • Precipitant & Protein Independent (either 8 + 1 or 16 + 1) • Differential Z • 300 uL loop and Extension

  22. HT Crystallography Options • Extended Head • 16 Channel synQUAD for Mother Liquors • Independent Z Axis for Protein Dispense

  23. New Products Honeybee Protein Crystallization Systems • Automated Gantry Descriptions • Nest • Based on ProSys platform • Twister Plate Handler • Actuated Plate Positions • Dual Wash Station • Protein Vial Holders • Extra Passive Plate Positions • Humidity Chamber • Digital Readouts

  24. New Products Honeybee Protein Crystallization Systems • Automated Gantry Descriptions • Dispense Head(s) • 9 mm Sitting Drop • 13 mm Hanging Drop • Wash Station Inserts • Vacuum Station Inserts • Precipitant & Protein Independent 16 + 1) • Differential Z (Sitting Drop) • 300 uL loop and Extension

  25. New Products 3 Dimensional Drawing of Automated Honeybee

  26. General Layout

  27. Magazine Lifter Assembly & Magazine

  28. Pick & Place Head

  29. Automated Honeybee Series

  30. Automated Honeybee Series • Microbatch, Hanging Drop, or Sitting Drop Application • Combinatorial Mixing • Overhead Gantry System • Pick & Place Slide Placement • Protein Chiller • Active Plate Holder • Twister Plate Handler

  31. Hanging Drop Video

  32. Overview Mother Liquors are dispensed via Hummingbird Technology onto various crystallization substrates Proteins are dispensed via synQUAD Hummingbird + synQUAD Technology

  33. Hummingbird Technology

  34. synQUAD Technology

  35. Conclusions • synQUAD technology supports miniaturization • synQUAD speed allows more wells/day • Hummingbird Technology supports parallel dispensing • All Non Contact

  36. Mm www.genomicsolutions.com

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