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Restriction Enzymes

DNA. Restriction enzymes. Restriction Enzymes. Heparan ase = enzyme. Problem: Is heparanase important in bone development? Hypothesis: If heparanase is overexpressed, then bone formation will abnormal.

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Restriction Enzymes

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  1. DNA Restriction enzymes Restriction Enzymes

  2. Heparanase = enzyme Problem: Is heparanase important in bone development? Hypothesis: If heparanase is overexpressed, then bone formation will abnormal.

  3. One way to find out, make a transgenic (can be an animal or plant that has a copy of the original gene which can be passed to offsrping) mouse that overexpresses heparanase only in the bones

  4. Nucleotides recognized by NotI Nucleotides recognized by ClaI Restriction Enzymes Heparanase

  5. RE B RE A Circular DNA Before Restriction Enzyme Digestion After Restriction Enzyme Digestion WithRestriction Enzyme Digestion RE B 2. 1. RE A Circular DNA 3. RE B RE A Linearized DNA

  6. Linearized DNA Agarose Gel: allows one to visualize DNA Circular DNA

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