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CLINICAL PATHOLOGY: C-Reactive Protein

CLINICAL PATHOLOGY: C-Reactive Protein. GENERAL OBJECTIVE : After finishing lab activity of C-RP, the student will be able to describe C-RP in the DMS case problem SPECIFIC OBJECTIVE : At the end of lab activity of C-RP, the student will be able to interprete: - the normal and abnormal C-RP.

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CLINICAL PATHOLOGY: C-Reactive Protein

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  1. CLINICAL PATHOLOGY:C-Reactive Protein GENERAL OBJECTIVE: After finishing lab activity of C-RP, the student will be able to describe C-RP in the DMS case problem SPECIFIC OBJECTIVE: At the end of lab activity of C-RP, the student will be able to interprete: - the normal and abnormal C-RP

  2. C-Reactive Protein • C-RP is a serum constituent originally define by its ability to precipitate Pneumococcus C polysaccharide • C-RP is an acute phase reactant: A protein synthesized by the liver as a non-specific response to the inflammation

  3. C-Reactive Protein • C-RP concentration increased in the serum of individu as a response to varies inflammatory condition and tissue necrosis • before specific antibody production starts and the ESR is increased. • After successful therapy, CRP level fall before the ESR decreases

  4. C-Reactive Protein • Specimen: serum • Reference range: 1. < 6 mg/L (aglutination method), semiquantitative 2. Immunoturbidimetric (quantitative) < 5 mg/L

  5. C-Reactive Protein Method: Latex agglutination Specimen: Serum Principle: Monoclonal anti C-RP will binds and agglutinate the CRP in the specimen

  6. PROCEDURE • Allow each component of reagents and specimen to reach room temperature • Gently shake the reagent • Place a drop of undiluted specimen to the slide • Add one drop of the latex reagent next to the drops of specimen • Spread the reagent and specimen over theentire area of test circle • Gently tilt the test slide backwards and forwards for two minutes. • Positive and negative controls should be included

  7. INTERPRETATION • Presence of agglutination indicates a level of C-RP in the specimen equal or > 6mg/L • The lack of agglutination indicates a C-RP level < 6 mg/L

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