Requirements of IBTO (AABB) Standards for an Antigen negative Blood Transfusion Request

1. Preparation of RBCs for Thalassemia patient with multiple antibodiesDetection and Identification of Alloantibodies to Red Cell AntigensIranian Blood Transfusion OrganizationMostafa Moghaddam MS, CLS ( ASCP ) BBHead of Immunohematology Reference LaboratoryISBT member of RARE Donor Working Party ISBT member of Immunohematology Working Party

2. Detection and Identification of unexpected Antibodies to Red Cell Antigensجستجو و شناسائي آنتي باديهاي غيرمنتظره عليه آنتي ژنهاي گلبول قرمز خون Requirements of IBTO (AABB) Standards for an Antigen negative Blood Transfusion Request 1.Positive identification of the recipient and the recipient blood samples. 2. ABO group and Rh (D) typing of the recipient's blood 3. Red cell antibody detection tests for clinically significant antibodies using the recipient’s serum/plasma ( Antibody Screening ) 4. Comparison of current findings on the recipient’s sample with records of previous results

3. Detection and Identification of unexpected Antibodies to Red Cell Antigensجستجو و شناسائي آنتي باديهاي غيرمنتظره عليه آنتي ژنهاي گلبول قرمز خون 5. Confirmation of the ABO group of red cell components . 6. Confirmation of the Rh type of Rh negative red cell components 7. Selection of components of ABO group / Rh type and appropriate antigen negative RBC unit for the recipient 8. Performance of a serologic or computer cross match 9. Labeling of products with the recipient’s identifying information

4. Detection and Identification of unexpected Antibodies to Red Cell Antigens • Clinically significant alloantibodies react at 37ºС or in the antihuman globulin (AHG) phase are usually IgG antibodies cause a transfusion reaction decrease survival of RBCs

5. Detection and Identification of unexpected Antibodies to Red Cell Antigensجستجو و شناسائي آنتي باديهاي غيرمنتظره عليه آنتي ژنهاي گلبول قرمز خون • Antibody Screen Definition : Testing the patient’s serum/ plasma with group ‘’O” reagent red cells in an effort to detect atypical antibodies

6. Detection and Identification of unexpected Antibodies to Red Cell Antigensجستجو و شناسائي آنتي باديهاي غيرمنتظره عليه آنتي ژنهاي گلبول قرمز خون

7. Automation Methods Tube technique - Gel technique Solid phase technique

9. More is no better in Blood Transfusion

10. Providing blood for Thalassemia patient with Anti-M & Anti-Jkband more A blood sample from a female patient with Hg 2.5 g/dl was received by IRL .Patient was suffering from serious multiple blood transfusion reactions including severe confusion and mild syncope.

11. Providing blood for Thalassemia patient

12. Patient’s Chronological Medical History

13. +Should Transfusion of thalassemia patients be matched using phenotype –matched RBC donor units?

14. +Should Transfusion of thalassemia patients be matched using phenotype –matched RBC donor units?

17. چرخه سازگـاری و خون سـالم = آزمایشگـاه رفرانس ایمونوهمـاتولوژی ImmunohematologyReference Laboratory = Compatibility Wheel and Safe Blood and if any one saved a life, it would be as if he saved the life of the whole people 32 maedeh