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Part II Lecture on Ig & TCR Genes 30 th October 2012

Part II Lecture on Ig & TCR Genes 30 th October 2012. by Mike Clark, PhD Reader in Therapeutic and Molecular Immunology Department of Pathology Division of Immunology Cambridge University UK CB2 1QP www.antibody.me.uk. Mammalian Antibody Classes.

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Part II Lecture on Ig & TCR Genes 30 th October 2012

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  1. Part II Lecture on Ig & TCR Genes30th October 2012 by Mike Clark, PhD Reader in Therapeutic and Molecular Immunology Department of Pathology Division of Immunology Cambridge University UK CB2 1QP www.antibody.me.uk

  2. Mammalian Antibody Classes • Antibodies are classed according to the type of immunoglobulin heavy chain • In addition each antibody class can have either κ (kappa) or λ (lambda) immunoglobulin light chains • In mammals we recognise the following classes and chains • IgM μ (mu) • IgD δ (delta) • IgG γ (gamma) • IgA α (alpha) • IgE ε (epsilon)

  3. Schematic view of IgG domains

  4. Kabat database variability of VH sequences Mouse VH Human VH

  5. Lysozyme CDR regions Antibody Fv

  6. Simplified view of a light chain locus

  7. Lymphocyte specific Enzymes used for Ig Gene repertoire AID Activation induced cytidine deaminase Class switching and somatic mutation RAG Recombination activation genes (RAG1 and RAG2) Segmental recombination

  8. AID LEUCINE REPEAT RECEPTORS Jawless fish Segmental recombination RAG IMMUNOGLOBULINS Somatic mutation Gene Rearrangement [V-(D)-J joining] Jawed fish Amphibians Class switch Gene Conversion Birds Marsupials Placentals Primates Slide courtesy of Michael Neuberger

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