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Biotechnology

Biotechnology. PCR (Polymerase chain reaction). What is it?. Restriction enzymes. What are they?. Gel Electrophoresis. What is it? How does it work? DNA is isolated DNA is copied with PCR Cut with restriction enzymes Run through charged gel box Compared. Comparing results.

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Biotechnology

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  1. Biotechnology

  2. PCR (Polymerase chain reaction) • What is it?

  3. Restriction enzymes • What are they?

  4. Gel Electrophoresis • What is it? • How does it work? • DNA is isolated • DNA is copied with PCR • Cut with restriction enzymes • Run through charged gel box • Compared

  5. Comparing results

  6. GMO’s (Genetically modified organisms) • What are they? • How are they made? • Chosen gene is cut with restriction enzymes, leaving “sticky ends” • Circular bacterial DNA is cut with same restriction enzymes, leaving “sticky ends” • Cut pieces are joined together, so bacteria now produces protein in gene

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