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UNIT 3. THE MICROBIAL WORLD. PROKARYOTES. Single-celled Oldest forms of life on earth Have cell wall Plasma/cell membrane NO nucleus and other membrane bound organelles Circular DNA Two prokaryotic domains: Bacteria and Archae. BACTERIA. 3 basic shapes: Cocci = round Bacilli= rod
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UNIT 3 THE MICROBIAL WORLD
PROKARYOTES • Single-celled • Oldest forms of life on earth • Have cell wall • Plasma/cell membrane • NO nucleus and other membrane bound organelles • Circular DNA • Two prokaryotic domains: Bacteria and Archae
BACTERIA • 3 basic shapes: • Cocci = round • Bacilli= rod • Spirilli =spiral • There is also a fourth shape- rings but not as common
Coccus Bacillus
Pelagibacter ubique - the most abundant life form on the planet - just recently discovered -found in open water
SYMBIOTIC BACTERIA • Bacteria that live in close association with other marine organisms
BIOLUMINESCENT BACTERIA • Bacteria that cause the light produced by some fish, squids, octopuses, and other animals of the deep- symbiotic relationship • Bacteria is sheltered in light-producing organs called PHOTOPHORES • Use the light to communicate, attract prey • Video 1 • Video 2
Chemosynthetic Bacteria • Symbiotic with mussels, clams, and tubeworms • Around hydrothermal vents • Manufacture organic matter from CO2 and the hydrogen sulfide from the vents • Live in the “feeding body” of the giant hydrothermal-vent tube worm Riftia
TETRODOTOXIN • Some symbiotic bacteria affect human health- such as the neurotoxin (tetrodotoxin) produced by symbiotic bacteria in pufferfish • One of the most powerful poisons, no anecdote • Found in the liver and gonads of the fish
Tetrodotoxin and other similar toxins are also found in flatworms, snails, crabs, sea stars and several species of fish • Also found in the blue-ringed octopus- very toxic animal
CYANOBACTERIA • “Blue-green” algae; photosynthetic • Contain chlorphyll a and a bluish pigment called PHYCOCYANIN • Most marine cyanobacteria also have a reddish pigment called PHYCOERYTHRIN • One of the first photosynthetic organisms on earth • Important in O2 production
STROMATOLITES- fossil calcareous mounds formed by cyanobacteria- 3 billion years old; still being formed
Cyanobacteria can tolerate wide ranges of salinity and temperature- • Endolithic= go into calcareous rocks and coral skeletons; form thick dark crusts on shoreline • Planktonic= multiply fast; change the color of the water • Epiphytes= photosynthetic bacteria that love on algae or plants • Endophytes= bacteria that live in algae
ARCHAE video • Simplest and primitive form of life • Important in the early evolution of life • Small and can by any of the three shapes of bacteria • MORE CLOSELY RELATED TO EUKARYOTES THAN BACTERIA • Can tolerate extreme environments- known as extremophiles but are not only found in these environments • Common in water and sediment environments
PROKARYOTIC METABOLISM • METABOLISM= all the chemical reactions that takes place in an organism • AUTOTROPHIC = make their own food; primary producers • Photoautotrophic= have chlorophyll or other pigments that capture sunlight and transfer it into organic compounds • Occurs in the bacterial membrane
CHEMOSYNTHETIC OR CHEMOAUTOTROPHIC= derive energy from other chemicals like hydrogen sulfide or other sulfur, nitrogen and iron compounds • Methanogens= produce methane (CH4)
HETEROTROPHS • Most marine bacteria • Cannot produce own food; obtain energy from respiration • Many are decomposers • Aerobic bacteria use oxygen; anaerobic bacteria do not require oxygen for respiration (thrive only when oxygen is not present)
some bottom-dwelling and planktonic cyanobacteria undergo nitrogen fixation where they convert nitrogen into ammonium which can be used a a source of nitrogen by primary producers
UNICELLULAR ALGAE • Mostly photosynthetic; have chloroplasts; eukaryotic • Lack flowers; have very simple reproductive structures; lack true leaves, stems and roots • Some show animal-like characteristics; move with a flagella; some consume food • considered protists
DIATOMS • PHYLUM- Heterokontophyta c • CLASS- Bacillarophyta • Unicellular • Cell walls made up of silicon (SiO2); this glass shell is known as a frastule • made up of two tightly fitted halves- looks like a box; different shapes • May have spines or ribs • Allows light to pass through so that the golden-brown chloroplasts can capture light • The perforations allow dissolved gases and nutrients to enter and exit
Diatoms cont… • Color caused by yellow and brown carotenoid pigments • Also has chlorophyll a and c • 12,000 species (half of these marine) • Most planktonic, but some have a stalk-like structure for attachment • Reproduce asexually by cell division • Frustules get progressively smaller
Diatom cont….. • Can also reproduce sexually • Resistant stages called auxospores- are larger diatoms; can be formed either asexually or through sexual reproduction • Rapid reproduction known as blooms • Deposits of frustules of dead diatoms forms a biogenous sediment of siliceaous material on the ocean floor; also known as diatomaceous ooze- forms diatomaceous earth which is used for filters in pools, for clarifying beer, as temp and sound insulators
DINOFLAGELLATES • Unicellular organisms • 2 flagella; one wrapped around a groove in the middle of the cell and one free • Cell wall made of “plates” of cellulose • Autotrophic and heterotrophic • All marine • Reproduce by cell division • Blooms form “Red Tides” • Some bioluminescent
Zooanthellae- dinoflagellate that are round, golden-brown • Live in close assoc. with some animals such as sponges, and sea anenomes • Important in reef corals because they fix carbon dioxide via photosynthesis and help build the coral skeleton (mine does not have the zooanthellae and that is why I need to supplement feed it shrimp)
Some dinoflagellates have life cycles with cyst stages and free-swimming larval stages • Pfiesteria- or the “phantom dinoflagellate” exists mostly as a cyst • Responsible for open sores on fish
Other Unicellular Algae • Silicoflagellates- star-shaped internal skeleton of silica; 2 flagella; common in marine sediments • Coccolithophorids- flagellated, spherical cells covered with button like structures called coccoliths made of calcium carbonate; found in sediments as fossils
PROTOZOANS- ANIMAL LIKE PROTISTS • Eukaryotic • Single-celled; some form colonies • Heterotrophic
Foraminiferans • Forams; have a shell or test made of calcium carbonate • Have pseudopodia (extensions of the cytoplasm); used for movement and obtaining of food • Live on bottom; free or attached • White Cliffs of Dover produced by foram sediments
RADIOLARIANS • Planktonic- in open water • Secrete shells made of glass (silica) • Usually spherical with spines • Pseudopodia captures food
Ciliates • Have cilia for locomotion and feeding • Paramecium • May be free living, attached to surfaces or within other organisms • Important food source
Fungi • Multicellular or unicellular (molds and yeasts) • 500 marine species • Decompose detritus • Some parasitic • Marine lichens