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Comparative Analysis of Chitinase Proteins in Pine Tree EST Sequences

Investigation of reference and putative chitinase proteins in jack and lodgepole pine trees using RT-PCR. Neighbor Joining method used for relationship estimation. Bootstrap values shown on consensus tree.

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Comparative Analysis of Chitinase Proteins in Pine Tree EST Sequences

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  1. Cluster 1 Class IV Cluster 6 Class V Cluster 5 Class III Cluster 4 Class II Cluster 2 Class II Cluster 3 Class I - II Supplemental Fig. S6. Topology of reference chitinase proteins and putative chitinase proteins derived from EST sequences from jack and lodgepole pine. Genes pointed by arrows were investigated by RT-PCR. Relationships were estimated using the Neighbor Joining method and the consensus tree was inferred from 1000 bootstrap replicates. Bootstrap values are shown for each branch. Pc – Pinuscontorta, Pb – Pinusbanksiana, Pm – Piceamariana, Pg – Piceaglauca, Pa – Piceabies, At – Arabiodpsis thaliana, Gh – Gossypiumhirsutum, St – Solanumtuberosum, Sc – Secale cereal, Zm –Zea mays, Hv – Hordeumvulgare, Nt – Nicotianatabaccum, Os –Oryzasativa, Sr – Sesbaniarostrata, Ms – Medicagosativa.

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