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Kelly O’Quinn Dr. Adam Melvin Cain Department of Chemical Engineering Louisiana State University

Harmful algal bloom (HAB)-on-a-chip: Development of a microfluidic platform to study algal chemotaxis. Kelly O’Quinn Dr. Adam Melvin Cain Department of Chemical Engineering Louisiana State University. Harmful Algal Blooms. Overabundance of algae Karenia brevis Problems Ecosystem

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Kelly O’Quinn Dr. Adam Melvin Cain Department of Chemical Engineering Louisiana State University

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  1. Harmful algal bloom (HAB)-on-a-chip: Development of a microfluidic platform to study algal chemotaxis Kelly O’Quinn Dr. Adam Melvin Cain Department of Chemical Engineering Louisiana State University

  2. Harmful Algal Blooms • Overabundance of algae • Kareniabrevis • Problems • Ecosystem • Economy • Health • Reproduction and Migration http://oceanservice.noaa.gov/hazards/hab/ http://naturalunseenhazards.wordpress.com/2012/08/01/washington-warns-of-paralytic-shellfish-poison-biotoxin-found-in-central-and-south-puget-sound-waters-lyme-disease-stories-presented-by-cdc-west-nile-virus-reports-from-ne-sc-tx-rabies-r/

  3. Migration of Algae • Phototaxis: upward migration toward light during day • Chemotaxis: downward migration toward nutrients at night

  4. Heterogeneous Environments • Algae migrate in response to many factors • Concentrations not constant throughout system

  5. Developing a New Technology • Microfluidic devices • Precise control of fluids • Laminar flow: diffusion-based mixing • Dynamic control • Past research • Homogeneous tanks • Other microfluidic devices http://www.rsc.org/ej/IB/2010/c0ib00055h/c0ib00055h-f1.gif

  6. Design and Fabricate the Device • Design in AutoCAD • Soft lithography • Master • PDMS replication

  7. Fabrication Spin coat photoresistonto wafer Place photomaskonto wafer then UV expose then bake Cross-linked polymers stay and non cross- linked dissolve in solution

  8. Device Assembly • Layers • PDMS • Agarose • Glass slide • Tubes • Options for adhering layers • Silanes • Thiol-ene resin • Plexiglas box • Negative pressure Shing-Yi Cheng, Steven Heilman, Max Wasserman, Shivaun Archer, Michael L. Shuler and Mingming Wu, “A hydrogel-based microfluidic device for the studies of directed cellmigration,” Lab Chip, 2007, 7, 763-769

  9. Characterize the Device • Ensure stable, well-controlled gradients formed • Fluorescent tracers • ImageJ line scan • COMSOL models

  10. Implement the Device for Studying Algae Migration • Chlamydomonasreinhardtii • Unicellular, survives in many environments • Load both channels • Allow gradient to develop • Load cells into center, flow-free channel

  11. Gathering and Analyzing Results • Chemotactic Index • Vector assigning • Design of Experiment (DOE) • Analysis of Variance (ANOVA)

  12. Future Applications • Better understanding of algae migration • Most prominent factors causing blooms • Developing new technology • Future collaborations with other labs

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