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Microscope Basics

Microscope Basics. (1) Body Tube. The long skinny tube that holds the eyepiece up above the microscope. It allows the light of the image to travel from the objective lenses to the eyepiece. (2) Nosepiece. It holds the objective lenses.

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Microscope Basics

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  1. Microscope Basics

  2. (1) Body Tube • The long skinny tube that holds the eyepiece up above the microscope. • It allows the light of the image to travel from the objective lenses to the eyepiece

  3. (2) Nosepiece • It holds the objective lenses. • It rotates around in a circle allowing you to choose which objective lens you want to use

  4. (3-5) Objective Lens • Attached to the nosepiece • Vary in size where 3 is the least powerful and 5 is the most powerful • Usually has different color bands to indicate its strength

  5. (6) Stage Clips • Shiny clips on the top of the stage • Holds the microscope slide in place

  6. (7) Diaphragm • Round disk that has several different size holes • Allows you to change the amount of light that comes up through the aperture

  7. (8) Light • The lamp shines light through the aperture and makes it easier to see the object

  8. (9) Eye Piece or Ocular Lens • Where you look into the microscope to see the image of the thing that you are looking at

  9. (10) Arm • Holds the upper portion of the microscope to the stage • It is the part where you carry the microscope

  10. (11) Stage • Flat area under the objective lenses that has a hole in the middle of it • Holds the glass slide or object you are investigating

  11. (12) Coarse Adjustment Knob • Big knob that allows you to move the stage closer to the upper portion of the microscope in order to bring the object into focus • Moves the stage up and down

  12. (13) Fine Adjustment Knob • Small knob under the coarse adjustment knob • Fine tunes the focus of the image • Moves the stage up & down by very small amounts

  13. (14) Base • Holds the microscope up

  14. (15) Power Switch • Turns the microscope on and off

  15. We can see better details with higher the powers of magnification, but we cannot see as much of the image. Which of these images would be viewed at a higher power of magnification? Comparing Powers of Magnification

  16. What’s my power? To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective. What are the powers of magnification for each of the objectives we have on our microscopes?

  17. 4 – Once you have the image in view, rotate the nosepiece to view it under different powers. Draw what you see on your worksheet! Be careful with the largest objective! Sometimes there is not enough room and you will not be able to use it! General Use Directions 1 - Turn the coarse adjustment knob towards you and lower the stage to the bottom. 2 – Turn on the microscope and then rotate the nosepiece to click the red-banded objective(lowest objective) into place. 3 – Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer. 5 – When you are done,turn offthe microscope. Put the dust cover back on. Clean off the slides that you used.

  18. 4 - Slowly lower the cover slip on top of the drop. Cover Slip Lower slowly 5 – Place the slide on the stage and view it first with the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives. You do not need to use the stage clips when viewing wet-mount slides! How to make a wet-mount slide … 1 – Get a clean slide and coverslip from your teacher. 2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess! 3 – Place the edge of the cover slip on one side of the water drop.

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