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ProStar 325 Detector

ProStar 325 Detector. Thursday, 2 January 2020. Petro van Poppel – Field Support Specialist. Overview. Introduction to PS 325 Detector Dual Wavelength Option LC Verify Software Flow cells Communications LC Verify Production Tests with 0x0 flow cell Wavescan Test (Mono Options)

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ProStar 325 Detector

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  1. ProStar 325 Detector Thursday, 2 January 2020 Petro van Poppel – Field Support Specialist

  2. Overview • Introduction to PS 325 Detector • Dual Wavelength Option • LC Verify Software • Flow cells • Communications • LC Verify • Production Tests with 0x0 flow cell • Wavescan Test (Mono Options) • Diagnostics Mode (Main Tab) • Electronics Test (Input/Output) • System Details (EEPROM) • Communications (COMM) • PS 335 Shipping Test • Problems

  3. Vis D2 325 Optics Module Light Path

  4. 325 Optics Ray Beam

  5. Dual Wavelength Option Dual wavelength is a firmware change that allows you to view 2 wavelengths “simultaneously” Galaxie Software LC Diagnostics Software

  6. 325/335 Back Panel P/N 02-101875-00 Relay Adaptor board I/O adaptor board for the sync signals P/N 02-101865-00

  7. Flow Cell Ray Beam General design of the 9x1 flow cell General design of the 9x0 flow cell

  8. Flow Cell Specs

  9. Communications • TCP/IP communications for Detector: • Static or Dynamic • Networked or standalone • Firewall • Access on Network, OS or PC

  10. LC Verify side

  11. LC Diagnostics Side Ctrl-Shift-F10 to enable Diagnostics side Ctrl-Shift-F9 to hide Diagnostics side

  12. PS 325 Test Certificate Test Certificate provided by factory • Parameters tested: • Noise • Wavelength Accuracy • Wavelength Reproducibility • Spectral Bandwidth Accuracy • AutoZero Precision Test • Detector Linearity Test

  13. LC Verify use in Final Testing

  14. PS 325 Noise Test (FT)

  15. PS 325 Wavelength Accuracy Test (FT)

  16. PS 325 Wavelength Repeatability Test (FT)

  17. PS 325 Spectral Bandwidth Test (FT)

  18. PS 325 Auto Precision Test (FT)

  19. PS 325 Detector Linearity Test (FT) pump water for at least 10 minutes at a pump head pressure of at least 100 atm using a back pressure restrictor of 40psi.

  20. PS 325 LCVerify Wavescan

  21. PS 325 Wavescan results

  22. PS 325 Contributions from each Lamp

  23. PS 325 LCVerify Diagnostics Mode

  24. PS 325 LCVerify Input/Output Test

  25. C A B PS 325 Main PWB Power Supply LED’s A: + 24 V power Supply B: + 9V and – 9V Power Supply C: + 5 V Power Supply Front of the Unit

  26. PS 325 LCVerify System Details

  27. PS 325 LCVerify Communications

  28. Caffeine Shipping Test • PS 325 Caffeine Shipping Test: • Test made up of 1 components: • Loop injection using 25 µg/ml caffeine • Test is conducted using Galaxie Software

  29. PS 325 Lamp Intensity Test Test 1: Lamp Intensities

  30. PS 325/335 Caffeine Loop Injection Test Test Calculates: Peak Height Peak Area Noise S/N

  31. Common Problems • High Noise • Lamp on, not ready • SIG_CC_ZERO_ORDER_PEAK_FIND_FAILED • SIG_CC_SIGNAL_OVERRANGE_REPREFORM_ LAMP_CAL • COM_CC_CIRCULAR_BUFFER_OVERRUN _OCCURED • 9555 LMP_CC_D2_FILAMENT_FAILURE • PROSTAR 325 DRIVER:  Collection not started - Acquisition time too short for the method. • Foggy Optics • PS 325 Error Messages Table can be found: • PS 325 Service Manual • Dealernet

  32. Foggy Optics • What is Foggy Optics?

  33. What is Foggy Optics? • Three types of Foggy optics • White powdery coating • Rust/brown spot burn • Speckled mirror (delamination)

  34. Foggy Optics • What is Foggy Optics? • Diagnosing Foggy Optics

  35. Diagnosing Foggy Optics • Run a “wavescan” test, • When bad • Open the optics and inspect.

  36. Wavescan test results Intensity for the 254 nm line Currently is 10 – 50 K counts

  37. False positive New D2 vs Aged D2 Intensities

  38. Foggy Optics • What is Foggy Optics? • Diagnosing Foggy Optics • Treating Foggy optics

  39. 0603 Project (Foggy Optics) • Proposed Fixes and their status: • White powder fogging: due to leaky flow cells and customer sample depositing on the mirrors • New Flow cells & fittings available for all cells except 4 x 0.15 • Brown powder fogging: due to UV attack on some of the foam parts close to the UV source. • Eliminating as much of the foam as possible and shielding the rest. • Mirror delamination:due to UV attack on the thin Al coating of the mirrors • Eliminated by putting a NiCr coating on the mirrors to strengthen the Al coating.

  40. Opening and cleaning the optics

  41. Cleaning 325 optics Clean area in circle Do not adjust!!

  42. Replacing Optics Wavelength calibration data must be entered into the 335 EEPROM

  43. Summary • Single and Dual Wavelength, UV and UV/vis • Optical path is open • Flow cell is dual path • Diagnostics Tests, like wavescan • Shipping Test • Main Problems (Foggy Optics)

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