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STARBIND I In vitro

STARBIND I In vitro. Measure conc. free toxin( ) at equilibrium in solution  2 µg/l Afl free. Toxin + binder  complex. Evaluating binders in vitro: example. Solution of toxins 10 µg/l Afl + 2 g/l binder  equilibrium. (pH-traject) .

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STARBIND I In vitro

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  1. STARBIND I In vitro

  2. Measure conc. free toxin( ) at equilibrium in solution  2 µg/l Aflfree Toxin + binder  complex Evaluating binders in vitro: example • Solution of toxins • 10 µg/l Afl + 2 g/l binder  equilibrium (pH-traject)  Afltotal: 10 µg/l Aflfree: 2 µg/l  Aflbound: 8 µg/l Afl  adsorption: 80%

  3. Clay minerals - 2003 Starbind Zearalenone 2000 mg/metric ton feed Clay minerals 5 kg/metric ton feed Clay minerals pH 2.5 Clinoptilolite pH 8.0 Yeast product Adsorption of 1040 mg ZEA/MT feed =52% Organic acids and salts

  4. Clay minerals & yeast - 2004 Starbind Aflatoxin 340 mg/metric ton feed Ochratoxin A 1000 mg/metric ton feed Clay min.& yeast 5 kg/metric ton feed Clay minerals pH 3.0 Clinoptilolite pH 6.5 Yeast product Adsorption of 230 mg aflatoxin/MT feed = 67% 302 mg ochratoxin A/MT feed = 30% Organic acids and salts

  5. Clinoptilolite - 2006 Starbind Aflatoxin 400 mg/metric ton feed Zearalenone 2000 mg/metric ton feed Clinoptilolite 10 kg/metric ton feed Clay minerals pH 3.0 Clinoptilolite pH 6.5 Yeast product Adsorption of 398 mg aflatoxin/MT feed = 99% 250 mg zearalenone/MT feed = 12.5% Organic acids and salts

  6. Starbind Clay minerals Clinoptilolite Yeast product Organic acids and salts Starbind in commercial feed Starbind 1kg/MT feed Aflatoxine diff. contamin Fumonisine. diff. contamin. pH 6.5 (3h) Adsorption of Aflatoxine ca 95% Fumonisine. ca 63%. Kasetsat University Thailand, 2008

  7. Screening project 2009

  8. Screening project 2009 - ZEA Percentage bound contamination (µg/L)

  9. Screening project 2009 - OTA Percentage bound contamination (µg/L)

  10. Screening project 2009 - AFL Percentage bound contamination (µg/L)

  11. Starbind I In vitro – adsorption isotherm

  12. Measurement of free toxin( ) at equilibrium In solution  2 µg/l AFfree Toxin + binder  complex Adsorption isotherm: example • Solution of toxins • 10 µg/l AF + 2 g/l binder  equilibrium (pH-traject)  AFtotal: 10 µg/l AFfree: 2 µg/l  AFbound: 8 µg/l AF  Per gram binder: 4 µg AF adsorption  Repeatment with the same dosage of toxin binder at higher concentration of toxin

  13. 12% 9% 24% 32% 70% 80% 90% Interpretation of adsorption-isotherm 28 16% 12% 33% 24 44% 20 80% 16 12 Bound toxin [µg/g binder] 80% 8 90% 4 0 0 40 60 70 80 90 10 20 30 50 150 160 170 190 100 110 120 130 140 180 200 Contamination [µg/kg] The steeper the slope  the bigger the affinity to the toxin The higher the saturation line  the higher the max. binding capacity

  14. Starbind Yeast product Organic acids and salts Isotherm voor Starbind: Zearalenone 0-5-10-50-150-500 ppb Starbind 2 kg/MT feed Binder G 2 kg/MT feed Clay minerals pH 3.0 (1h) pH 3.0 (1h) Clinoptilolite pH 6.5 (3h) Isotherm

  15. ZEA binding Adsorption isotherm: Binder Starbind Binder G 300 250 200 150 bound zearalenone [ppb] 100 50 0 0 50 100 150 200 250 300 350 400 450 500 free zearalenone [ppb] Conclusion:  The Starbind isotherm shows a steeper slope  higher affinity to ZEA  Binder G shows a saturating slope

  16. ZEA binding Binder Starbind Binder G 500 450 400 350 300 Free zearalenone [ppb] 250 200 150 100 50 0 0 50 100 150 200 250 300 350 400 450 500 550 contamination [ppb] Conclusion: Starbind can bind more ZEA Starbind can reduce a higher contamination (120 ppb) to a safe level than binder G

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