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Visual Sampling of Fishes. Introduction to Sampling Visual Sampling Methods Evaluation of Effectiveness. GOAL: Describe Complex Biological Communities. PROBLEM: Complete census is impossible!!. APPROACH: ‘sub-sample’ several units. Progressive levels of sampling effort.
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Visual Sampling of Fishes • Introduction to Sampling • Visual Sampling Methods • Evaluation of Effectiveness
GOAL: Describe Complex Biological Communities PROBLEM: Complete census is impossible!! APPROACH: ‘sub-sample’ several units
Progressive levels of sampling effort. Time (= cost) increases with each level Qualitative - estimates species present, no abundance information Semi-quantitative - estimates species present andrelative abundance Quantitative - estimates species present and densities
Random vs Fixed Stations Random sampling:addresses some parametric test assumptions Fixed-station sampling:greater power when funding limited
Spatial and Temporal Considerations Many projects sample at established: time of day, lunar cycle, tidal cycle... Sampling day and night, or among seasons Distributional studies sample randomly through space
1. Qualitative Description • Presence/absence only – no abundance estimates • Example: Timed Swim • establish specified time • haphazard swim • record species observed
Species-site Checklist REA Fish Survey Depth/habitat distributions Island checklist of species New records, range extensions
2. Semi-Quantitative DescriptionRapid Visual Transect (RVT) Relative abundances estimated Species-time relationships Commoner species sighted before rarer species
Rapid Visual Transect • Establish time survey duration • (e.g., 0.5 hrs, 1.0 hrs, etc.). • Divide survey period into sub-intervals • (e.g., 5 or 10 mins). 3. Surveyor swims freely within sampling habitat.
Each species is recorded once • In the time interval first seen.
6. Species assigned scores based on time seen: TimeIntervalScore 00:00-04:59 1 5 05:00-09:59 2 4 10:00-14:59 3 3 15:00-19:59 4 2 20:00-24:59 5 1 7. Scores from multiple surveys are averaged. • Rank mean scores • (higher score = common; lower score = rare).
3. Quantitative Description 1. Densities are estimated 2. Sampler records: • a. species observed • b. number of individuals of each species • c. size/position of each individual (optional)
Quantitative Descriptions Methods taught at QUEST: Strip Transect (SST) Stationary Point Count (Circular Plot - CPLOT)
Strip Transect (SST) Deploy transect line Record species and number (and size) in assigned segment
In QUEST: four 25 m transects (paired) sampled per team 4 m 2 m 4 m 25 m 25 m 2 m 50 m transect line
Fish Belt-Transect - NOAA Program Method • Fish identified to “species” & counted • Swim-out: record fish >20 cm in 4m wide swath • Swim-back: record fish <20 cm in 2m wide swath Transect 2 25 m Diver 2 2m • Common species listed • Enter number and • size of each Transect 1 25 m Diver 1
Stationary Point Count Circular Plot – ‘C-PLOT’ Popular in Caribbean Plots usually chosen randomly or haphazardly In QUEST: Transect line is deployed Team waits for fishes to settle Then proceeds to pre-determined ‘point’
Record all fishes within acylinder set radius height above bottom (at QUEST r = 5 m, h = 1 m) r Cylinders cannot overlap Each cylinder treated as a replicate
Fish Stationary Point Count (SPC) • 4, 15-m diameter “cylinders”, 12-15m deep • 1st , 5 min. – create a list of species observed; • NO counts or sizes recorded. • After 5 min. – divers count & size individuals 7.5m radius circle 7.5m radius circle 30m transect Diver 2 Diver 1
END Multiple methods are used in some Programs
A. Principal Advantages 1. least disruptive and biased method for surveying fishes (compared to ???) 2. fairly inexpensive
Principal disadvantage: • some species chronically under-estimated: 1. large, mobile species (jacks, sharks).
Principal disadvantage: • some species chronically under-estimated: • schooling fishes with patchy distributions • (small jacks, mullet, some goatfishes).
Principal disadvantage: • some species chronically under-estimated: • cryptic species • (cardinalfishes, eels, gobies, blennies).
Principal disadvantage: • some species chronically under-estimated: 4. rare fishes
2. Swimming speed affects accuracy • at fast speeds, uncommon species • overlooked. b. at slow speeds, mobile species overestimated. c. rare species overlooked at all speeds.