'5 phosphate' presentation slideshows

DNA Endonucleases

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Nucleic acids and enzymes

Lecture 3. Nucleic acids and enzymes. The nuts and bolts. Most by David Tscharke @ RSB. Lecture overview. How to ‘ read ’ DNA Different types of nucleic acids Cutting DNA by restriction enzymes Synthetic DNA Enzymes used in molecular biology

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DNA Topology DNA has to be coiled to fit inside the cell

DNA Topology DNA has to be coiled to fit inside the cell. DNA polymers must be folded to fit into the cell or nucleus (tertiary structure). DNA Topology :. DNA Topology: linking number. Consider a 260 bp B-duplex:. Connect the ends to make a circular DNA:. Tw = 260/10.4 = 25.

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Carbohydrate Biosynthesis in Plants

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ENZYMES THAT MODIFY DNA AND RNA 1. RESTRICTION ENDONUCLEASES AND METHYLASES

ENZYMES THAT MODIFY DNA AND RNA 1. RESTRICTION ENDONUCLEASES AND METHYLASES RESTRICTION ENDONUCLEASES EXIST IN NATURE IN PROKARYOTES

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Pentose phosphate pathway

Pentose phosphate pathway. Pentose phosphate pathway has two phases. The main product of PPP is ribose 5-phosphate and NADPH. PPP oxidizes glucose 6-phosphate, producing ribose 5-phosphate (precursor for nucleotides) and NADPH (reducing agent for lipid biosynthesis).

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Phosphoenolpyruvate (PEP) condenses with erythrose 4-phosphate to form a 7-carbon compound,

Phosphoenolpyruvate (PEP) condenses with erythrose 4-phosphate to form a 7-carbon compound, 2-keto 3-deoxy arabinoheptulosonate, DAHP. DAHP cyclises to form 5-dehydroquinate. 5-dehydroquinate. Dehydration and reduction yield shikimate. Shikimate. Quinate. EPSPS.

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Study on the performance condition optimization of analytic method for p-chlorophenol compounds by high performance liqu

Little universal method is monitored p-chlorophenols compounds in environmental media. In the paper, analytic method of p-chlorophenols compound by high performance liquid chromatography (HPLC) was optimized. The result were that the mobile phase was 0.5% phosphoric acid and acetonitrile, and flow rate was 1.5mL/min. The column temperature of HPLC was 30℃, and the injection volume was 10μL, detection wavelength was 218nm. In monitoring process, the gradient elution mode determination of pentachlorophenol was used. In this condition, the pentachlorophenol, o-chlorophenol, m-chlorophenol and phenol could be seperated very well, the standard cures were Y=68.523x 360.08, Y=18.392x 17.575, Y=20.09x 16.319, and Y=26.379x 17.061, respectively. And the relative standard deviation (RSD) was 0.83%, 1.04%, 0.91%, 0.40%, respectively. The average recovery of the added index were from 89.17~100.58%.At last, sample of pentachlorophenol wastewater after biodegradation was monitored by HPLC in accordance with the optimization of the chromatographic conditions.. The result indicated that there was pentachlorophenol 12.35mg/L, p-chlorophenol 6.75mg/L, o-chlorophenol 3.24 mg/L, phenol 1.79mg

58 views • 4 slides