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T7 terminator

Fig. S1. Pst I digestion + ligase. Pst I. Pst I. T7-TopRI-5. T7-TopRI-4. Pst I. Pst I. Pst I. Pst I. PCR 4 + 5. T T7. T T7. Bam HI. Bgl II. pET-9d. T7 terminator. T T7. P T7. ATG. pQET7-TopRI. megaprimer. T7-TopRI-3. Col E1. Sac I. Xho I. Sac I. Xho I. PCR 3 + mega.

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T7 terminator